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Updated: Jul 23, 2026

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Published on: March 25, 2009
Development of a two-photon fluorescent probe for imaging hydrogen sulfide (H2S) in living cells and zebrafish
Chen Xu1, Yukun Zhang1, Hui Sun1
1State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Pulp & Paper Science and Technology of Shandong Province/Ministry of Education, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China. rmg888@qlu.edu.cn.
Abstract:
We present a new two-photon fluorescent probe (T-HS) for the detection of H2S. With the addition of hydrogen sulfide, the absorption and fluorescence spectra of the probe show regular changes. The probe exhibited favorable properties, such as large turn-on fluorescence signal, good selectivity and low cytotoxicity. Moreover, the probe T-HS was successfully used for the fluorescence imaging of H2S in live cells and zebrafish.

