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Distinctive features of lipoprotein profiles in stroke patients
Tomokazu Konishi1, Yurie Hayashi1, Risako Fujiwara2
1Graduate School of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
Plos One
|April 5, 2023
Summary
Lipoprotein profiles analyzed by HPLC effectively distinguish stroke patients from controls. Glycerol levels and the TG/cholesterol ratio in chylomicrons show potential as new biomarkers for atherosclerotic diseases.
Area of Science:
- Cardiovascular Science
- Biochemistry
- Medical Diagnostics
Background:
- Lipoprotein profiles are crucial for preventing atherosclerotic diseases.
- Traditional methods like ultracentrifugation and enzymatic assays have limitations in accuracy.
- High-performance liquid chromatography (HPLC) offers a viable alternative for lipoprotein analysis.
Purpose of the Study:
- To compare HPLC-derived lipoprotein profiles between stroke patients and controls using data-driven analysis.
- To identify novel biomarkers for atherosclerotic disease risk.
- To evaluate the efficacy of current screening methods and treatments.
Main Methods:
- Gel filtration HPLC was used to analyze lipoprotein profiles.
- Data-driven analyses were applied to HPLC data from stroke patients and healthy controls.
- Lipoprotein levels, ratios (e.g., TG/cholesterol), and free glycerol were assessed.
Main Results:
- HPLC data successfully separated stroke patients from controls.
- Low levels of HDL1 (high-density lipoprotein 1) were observed in many patients.
- A low TG/cholesterol ratio in chylomicrons was characteristic of patients, while high free glycerol was noted in the elderly.
- LDL cholesterol was not found to be a significant risk factor in this study.
- Statins showed minimal impact on the analyzed lipoprotein factors.
Conclusions:
- HPLC-based lipoprotein profiling is a promising tool for distinguishing atherosclerotic disease states.
- Free glycerol and chylomicron TG/cholesterol ratio may serve as adaptable and valuable clinical indicators.
- Current screening methods and treatment guidelines may require revision based on these findings.
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