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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

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Characterization and Optimization of Injectable In Situ Crosslinked Chitosan-Genipin Hydrogels.

Tyler R Priddy-Arrington¹, Reagan E Edwards¹, Claire E Colley¹

¹Department of Biomedical Engineering, Louisiana Tech University, Ruston, LA, 71272, USA.

Macromolecular Bioscience

|April 5, 2023

Summary

Researchers created a new injectable chitosan-genipin hydrogel that crosslinks at body temperature. This mechanically robust and biocompatible hydrogel offers a promising solution for minimally invasive biomedical applications.

Keywords:

chitosan genipin in situ crosslinking injectable hydrogels

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Published on: October 26, 2016

Area of Science:

Biomaterials Science
Polymer Chemistry
Tissue Engineering

Background:

Injectable hydrogels are increasingly important for minimally invasive medical applications.
Existing chitosan hydrogels face trade-offs between mechanical strength, biocompatibility, and degradation rate.
Toxic crosslinking agents or insufficient crosslinking limit current chitosan hydrogel applications.

Purpose of the Study:

To develop and characterize a thermally-driven, injectable chitosan-genipin hydrogel for in situ crosslinking at 37°C.
To create a hydrogel that is mechanically robust, biodegradable, and highly biocompatible.
To explore the potential of natural crosslinkers for improved hydrogel properties.

Main Methods:

Characterization of crosslinking kinetics, injectability, and viscoelastic properties.
Assessment of swelling behavior and pH responsiveness.
Evaluation of biocompatibility using human keratinocyte cells over 7 days.

Main Results:

Chitosan-genipin hydrogels successfully crosslinked in situ at 37°C, showing temperature sensitivity.
Hydrogels exhibited mechanical stability with sustained swelling over weeks before biodegradation.
Excellent long-term cell viability confirmed high biocompatibility, even during crosslinking.

Conclusions:

The developed chitosan-genipin hydrogel demonstrates desirable properties for biomedical use.
Its injectable and in situ crosslinking capabilities make it suitable for minimally invasive applications.
This natural crosslinker-based hydrogel offers a promising alternative to existing materials.