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Related Experiment Video

Updated: Aug 4, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
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MnO4--Triggered Immediate-Stable Real-Time Fluorescence Immunosensor with High Response Speed and Low Steady-State

Wenlin Ma1, Lihua Pang1, Jinhua Liu1

  • 1Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, China.

Analytical Chemistry
|April 5, 2023
PubMed
Summary

A new real-time fluorescent immunosensor offers rapid and stable detection for health and environmental monitoring. This innovative sensor uses a unique chemical reaction for highly sensitive detection of dopamine and alkaline phosphates, proving effective in clinical samples.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Materials Science

Background:

  • Real-time chemical and biological sensing is crucial for health and environmental monitoring.
  • Existing detection methods often lack the required speed and stability.
  • There is a pressing need for advanced sensing technologies.

Purpose of the Study:

  • To develop an immediate-stable, real-time fluorescent immunosensor with high response speed and minimal error.
  • To utilize a novel MnO4−-triggered fluorogenic reaction for sensitive biomolecule detection.
  • To demonstrate the sensor's applicability in clinical diagnostics.

Main Methods:

  • Construction of a fluorescent immunosensor based on the reaction between dopamine and orcinol monohydrate.
  • Characterization of the product (azamonardine) using mass spectrometry and NMR spectroscopy.
  • Application of the sensor for detecting dopamine, alkaline phosphates (ALP), and cardiac troponin I (cTnI).

Main Results:

  • The sensor exhibits a response speed of ~100% in less than 1 second with near-zero steady-state error.
  • Achieved limits of detection: 10 nM for dopamine, 0.1 mU/mL for ALP, and 0.05 ng/mL for cTnI.
  • Successful application in detecting cTnI in clinical serum samples, with results comparable to commercial ELISA.

Conclusions:

  • The developed sensor provides an immediate-stable, real-time fluorescent detection platform.
  • This technology offers a promising approach for trace biomolecule detection in clinical diagnosis.
  • The sensor demonstrates high sensitivity, stability, and real-time capabilities.