MnO₄^--Triggered Immediate-Stable Real-Time Fluorescence Immunosensor with High Response Speed and Low Steady-State...

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Materials Science

Background:

• Real-time chemical and biological sensing is crucial for health and environmental monitoring.
• Existing detection methods often lack the required speed and stability.
• There is a pressing need for advanced sensing technologies.

Purpose of the Study:

• To develop an immediate-stable, real-time fluorescent immunosensor with high response speed and minimal error.
• To utilize a novel MnO4−-triggered fluorogenic reaction for sensitive biomolecule detection.
• To demonstrate the sensor's applicability in clinical diagnostics.

Main Methods:

• Construction of a fluorescent immunosensor based on the reaction between dopamine and orcinol monohydrate.
• Characterization of the product (azamonardine) using mass spectrometry and NMR spectroscopy.
• Application of the sensor for detecting dopamine, alkaline phosphates (ALP), and cardiac troponin I (cTnI).

Main Results:

• The sensor exhibits a response speed of ~100% in less than 1 second with near-zero steady-state error.
• Achieved limits of detection: 10 nM for dopamine, 0.1 mU/mL for ALP, and 0.05 ng/mL for cTnI.
• Successful application in detecting cTnI in clinical serum samples, with results comparable to commercial ELISA.

Conclusions:

• The developed sensor provides an immediate-stable, real-time fluorescent detection platform.
• This technology offers a promising approach for trace biomolecule detection in clinical diagnosis.
• The sensor demonstrates high sensitivity, stability, and real-time capabilities.