Association of maternal folate and B₁₂ vitamin status with gestational diabetes mellitus: Still an open issue

There is conflicting evidence about the role of folate and B₁₂in gestational diabetes mellitus (GDM) onset. The association of vitamin status with GDM was therefore revalued, also measuring the B₁₂active form holotranscobalamin.

677 women were evaluated at 24-28 weeks of gestation when OGTT was carried out. The 'one-step' strategy was employed for GDM diagnosis. Odds ratio (OR) of having GDM was estimated to quantify the association with vitamin levels.

180 women (26.6%) had GDM. They were older (median, 34.6 vs. 33.3 years, p = 0.019) and had higher body mass index (BMI) (25.8 vs. 24.1 kg/m², p < 0.001). Multiparous women had lower levels of all evaluated micronutrients, while overweight lowered both folate and total B₁₂, but not holotranscobalamin. Lower total B₁₂(270 vs. 290 ng/L, p = 0.005), but not holotranscobalamin, was observed in GDM, being weakly negatively correlated with fasting glycemia (r = -0.11, p = 0.005) and 1-h OGTT serum insulin (r = -0.09, p = 0.014). At multivariate analysis, age, BMI and multiparity remained the strongest GDM predictors, while total B₁₂(but not holotranscobalamin and folate) showed a slight protective effect (OR = 0.996, p = 0.038).

A weak association between total B₁₂ levels and GDM risk was shown, but it was not confirmed when holotranscobalamin was measured.