Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.2K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Unified Platform for Direct Alkynylation from Alcohols via Deoxygenative Metallaphotoredox Catalysis.

Journal of the American Chemical Society·2026
Same author

High-Precision Intrinsic Interactome Elucidation of Chimeric Antigen Receptors via Photocatalytic Micromapping (μMap-CAR).

Journal of the American Chemical Society·2026
Same author

Piecewise Stereoselective Assembly of Multisubstituted Alkenes.

Journal of the American Chemical Society·2026
Same author

Oxyalkylation of Alkenes via Triple Radical Sorting.

Journal of the American Chemical Society·2026
Same author

Structure of ergosteryl-aspartate synthase reveals how an entrapped tRNA is used like a prosthetic swinging arm in the synthesis of aminoacylated sterols.

Nature communications·2026
Same author

Chemical biology tools for studying histone post-translational modifications.

Cell chemical biology·2026

Related Experiment Video

Updated: Aug 4, 2025

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.2K

Tracking chromatin state changes using nanoscale photo-proximity labelling.

Ciaran P Seath1,2,3, Antony J Burton2,4, Xuemeng Sun2

  • 1Merck Center for Catalysis at Princeton University, Princeton, NJ, USA.

Nature
|April 5, 2023
PubMed
Summary

We developed µMap, a nanoscale proximity-labeling method, to map nuclear protein-protein interactions. This technique reveals how these interactions change with cancer mutations and drug treatments, advancing epigenetic drug discovery.

More Related Videos

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.3K
Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
21:55

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

30.8K

Related Experiment Videos

Last Updated: Aug 4, 2025

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.2K
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.3K
Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
21:55

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

30.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Cellular processes rely on biomolecular interactions, crucial for cell fate.
  • Altered interactions can lead to disease or therapeutic effects.
  • Mapping nuclear protein-protein interactions is challenging due to low abundance, transient binding, and technology limitations.

Purpose of the Study:

  • To develop a novel method for mapping nuclear protein-protein interactions.
  • To investigate changes in nuclear interactomes in response to cancer mutations and drug treatments.

Main Methods:

  • Traceless incorporation of iridium-photosensitizers into the nucleus using engineered split inteins.
  • Activation of diazirine warheads via Dexter energy transfer to form reactive carbenes.
  • Nanoscale proximity-labeling (µMap) for cross-linking and quantitative chemoproteomics analysis.

Main Results:

  • µMap successfully maps nuclear protein-protein interactions within a ~10 nm radius.
  • The method revealed critical changes in interactomes associated with cancer mutations.
  • µMap identified altered interactions upon treatment with small-molecule inhibitors.

Conclusions:

  • µMap provides a powerful tool for understanding nuclear protein-protein interactions.
  • This technique enhances the fundamental understanding of nuclear micro-environments.
  • µMap is expected to significantly impact epigenetic drug discovery in academia and industry.