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Urinary complement profile in IgA nephropathy and its correlation with the clinical and pathological characteristics
Dongqing Wang1, Changwei Wu1, Sipei Chen1
1Renal Department and Nephrology Institute, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Frontiers in Immunology
|April 6, 2023
Summary
Urinary complement levels are elevated in IgA nephropathy (IgAN), correlating with kidney damage and reduced function. This suggests complement pathways could be therapeutic targets for IgAN patients.
Area of Science:
- Nephrology
- Immunology
- Proteomics
Background:
- The role of the complement system in IgA nephropathy (IgAN) pathogenesis remains incompletely understood.
- Investigating urinary complement profiles offers insights into IgAN pathophysiology.
Purpose of the Study:
- To characterize the urinary complement profile in IgA nephropathy (IgAN) patients.
- To explore correlations between urinary complement components and clinical/pathological features of IgAN.
Main Methods:
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for urinary protein analysis in IgAN, membranous nephropathy (MN), and healthy controls (HC).
- Bioinformatic analyses (GO, KEGG) to identify differentially expressed proteins and pathways.
- Enzyme-linked immunosorbent assay (ELISA) for validation of specific complement components.
Main Results:
- Significant enrichment of complement proteins in the urine of IgAN patients compared to HC.
- Urinary complement protein abundance correlated with estimated glomerular filtration rate (eGFR).
- Specific complement proteins (collectin12, CFH, CFHR2, CFB, C8g, C9) showed positive correlations with markers of renal dysfunction and histological damage (serum creatinine, glomerulosclerosis, tubular atrophy/interstitial fibrosis).
Conclusions:
- Abnormally elevated urinary complement components are significantly associated with impaired renal function and histological damage in IgAN.
- Urinary complement profiles may serve as potential biomarkers for IgAN monitoring.
- These findings suggest the complement system as a potential therapeutic target for IgAN.
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