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Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
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Neutral natural deep eutectic solvents as anti-biofilm agents
Helene Liepelt Nystedt1, Krister Gjestvang Grønlien1, Rebekka Rekkedal Rolfsnes2
1Section for Pharmaceutics and Social Pharmacy, Department of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, NO-0316, Oslo, Norway.
Biofilm
|April 6, 2023
Summary
Natural deep eutectic solvents (NADES) show promise as anti-biofilm agents. Two NADES, choline chloride:xylitol and choline chloride:glycerol, significantly reduced viable bacterial cells in biofilms.
Area of Science:
- Biochemistry and Pharmaceutical Sciences
- Microbiology and Infectious Diseases
Background:
- Natural deep eutectic solvents (NADES) are biodegradable, low-toxicity liquids with versatile applications.
- NADES' ability to interact with biological macromolecules suggests potential as anti-biofilm agents.
Purpose of the Study:
- To investigate the anti-biofilm efficacy of three neutral pH NADES against Staphylococcus aureus and Pseudomonas aeruginosa.
- To evaluate the capacity of NADES to disrupt existing biofilm matrices and eradicate biofilm-forming bacteria.
Main Methods:
- Three NADES (choline chloride:xylitol, choline chloride:glycerol, betaine:sucrose) were tested against pre-formed biofilms of S. aureus and P. aeruginosa.
- Biofilm biomass removal and reduction in viable cell counts were quantified.
- Inhibition of de novo biofilm formation and planktonic growth were assessed.
Main Results:
- Choline chloride:xylitol (ChX) and choline chloride:glycerol (ChG) reduced viable cells in pre-formed biofilms by 4-6 orders of magnitude.
- All tested NADES achieved significant biofilm biomass removal (27-67% for S. aureus, 34-49% for P. aeruginosa).
- NADES inhibited biofilm formation at sub-MIC concentrations, potentially by affecting planktonic growth.
Conclusions:
- Neutral NADES exhibit significant anti-biofilm activity against S. aureus and P. aeruginosa.
- NADES demonstrate potential for use in pharmaceutical formulations to combat bacterial biofilms.
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