Related Experiment Video
Updated: Aug 4, 2025

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
Yellow emissive nitrogen-doped carbon dots as a fluorescence probe for the sensitive and selective detection of
1Key Laboratory of Applied Chemistry and Nanotechnology at Universities of Jilin Province, Changchun University of Science and Technology Changchun 130022 China houjuan0503@126.com.
Abstract:
In this work, yellow emissive carbon dots (Y-CDs) were prepared via a simple hydrothermal method using catechol and hydrazine hydrate as the carbon and nitrogen sources, respectively. The average particle size was 2.99 nm. The Y-CDs demonstrate excitation-dependent emission properties, and the maximum emission wavelength is 570 nm at E x = 420 nm. The fluorescence quantum yield is calculated to be 28.2%. Ag+ could quench the fluorescence of Y-CDs with high selectivity. The quenching mechanism was further explored by various characterization techniques. A sensitive fluorescent probe for Ag+ detection was established based on Y-CDs with a linear range of 3-300 μM. The detection limit was calculated to be 1.1 μM. The proposed method shows satisfactory results in real water samples without interference by coexistence.

