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Fluorescent indicator displacement assay for the discovery of UGGAA repeat-targeted small molecules
Tomonori Shibata1, Yasumasa Matsumoto2, Akiko Iihara2
1Department of Regulatory Bioorganic Chemistry, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka 567-0047, Japan. shibata@sanken.osaka-u.ac.jp.
Chemical Communications (Cambridge, England)
|April 6, 2023
Abstract:
We report that a selective fluorescent indicator NBD-NCD for UGGAA repeats resulted in fluorescence quenching upon binding to RNA and recovered the fluorescence by displacing NBD-NCD with UGGAA repeat-targeted small molecules. The fluorescent indicator displacement assay using NBD-NCD can detect the interaction of small molecules with UGGAA repeats.
