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An improved procedure for obtaining germ-free laboratory mice
Laboratory Animals
|April 1, 1986
Summary
Using intermediate isolators improves germ-free mouse production. This technique ensures only viable, contamination-free litters are transferred to larger isolators, saving time and effort.
Area of Science:
- Animal models
- Microbiology
- Laboratory techniques
Background:
- Laminar air-flow cabinets are established for producing germ-free mice.
- Existing methods require significant time and effort.
Purpose of the Study:
- To present a modified technique for germ-free mouse production.
- To improve the efficiency and reliability of germ-free mouse generation.
Main Methods:
- Utilizing small intermediate isolators in conjunction with laminar air-flow cabinets.
- Implementing a selection process for litter viability and contamination status before transfer.
Main Results:
- Successful transfer of only viable and demonstrably contamination-free litters.
- Enhanced economy of time and effort in germ-free mouse production.
Conclusions:
- The modified technique using intermediate isolators is effective.
- This method optimizes germ-free mouse generation by ensuring litter quality and reducing contamination risk.