Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120.

Cell proliferation·2023

Same author

Ustilaginoidea virens-secreted effector Uv1809 suppresses rice immunity by enhancing OsSRT2-mediated histone deacetylation.

Plant biotechnology journal·2023

Same author

The Interaction of Dietary Patterns and Genetic Variants on the Risk of Cardiovascular Diseases in Chinese Patients with Type 2 Diabetes.

Molecular nutrition & food research·2023

Same author

Birth spacing and risk of adverse pregnancy and birth outcomes: A systematic review and dose-response meta-analysis.

Acta obstetricia et gynecologica Scandinavica·2023

Same author

Identification of propranolol and derivatives that are chemical inhibitors of phosphatidate phosphatase as potential broad-spectrum fungicides.

Plant communications·2023

Same author

Cancer Cell Membrane-Coated Nanoparticle Co-loaded with Photosensitizer and Toll-like Receptor 7 Agonist for the Enhancement of Combined Tumor Immunotherapy.

ACS nano·2023

Search research articles

Related Experiment Video

Updated: Aug 4, 2025

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

Comparative physiological and proteomic response to phosphate deficiency between two wheat genotypes differing in

Lu Zheng¹, Ruonan Wang², Peijun Zhou²

¹State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

Journal of Proteomics

|April 6, 2023

Summary

Wheat genotypes show genetic variation in phosphorus utilization efficiency (PUE). The PUE-efficient genotype TM98 maintains energy metabolism and enhances specific protein pathways under phosphate deficiency, unlike H4399.

Keywords:

Genetic variation Glutathione metabolism Phosphate deficiency Phosphate signaling pathway Phosphorus utilization efficiency Sulfolipid biosynthesis

More Related Videos

A Simple Protocol for Mapping the Plant Root System Architecture Traits

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

Author Spotlight: Unraveling Plant Responses to Abiotic Stresses Using the PlantScreen Robotic Platform

Author Spotlight: Unraveling Plant Responses to Abiotic Stresses Using the PlantScreen Robotic Platform

Published on: June 7, 2024

Related Experiment Videos

Last Updated: Aug 4, 2025

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

A Simple Protocol for Mapping the Plant Root System Architecture Traits

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

Author Spotlight: Unraveling Plant Responses to Abiotic Stresses Using the PlantScreen Robotic Platform

Author Spotlight: Unraveling Plant Responses to Abiotic Stresses Using the PlantScreen Robotic Platform

Published on: June 7, 2024

Area of Science:

Plant Physiology
Molecular Biology
Agricultural Science

Background:

Phosphorus utilization efficiency (PUE) varies significantly among wheat genotypes.
Understanding the genetic and molecular mechanisms of PUE is crucial for sustainable agriculture.

Purpose of the Study:

To investigate the physiological and proteomic differences between wheat genotypes with contrasting PUE under phosphate deficiency.
To identify key molecular pathways contributing to high PUE in wheat.

Main Methods:

Screening of 17 bread wheat genotypes for PUE based on shoot soluble phosphate concentrations.
Label-free quantitative proteomic analysis of shoots from contrasting genotypes (Heng4399 and Tanmai98) under phosphate deficiency.
Analysis of gene expression in the PHR1-centered phosphate signaling pathway.

Main Results:

The TM98 genotype exhibited significantly higher PUE than H4399, particularly under phosphate deficiency.
Phosphate deficiency impacted protein abundance related to nitrogen and phosphorus metabolism, small molecule, and carboxylic acid metabolism in both genotypes.
TM98 maintained protein abundance in energy metabolism and photosynthesis, while H4399 showed a decrease. TM98 also accumulated proteins involved in pyruvate metabolism, glutathione metabolism, and sulfolipid biosynthesis.

Conclusions:

The PUE-efficient genotype TM98 effectively utilizes the PHR1-centered phosphate signaling pathway.
TM98's ability to maintain energy metabolism and enhance specific metabolic pathways under phosphate deficiency contributes to its superior PUE.
Identified differentially expressed proteins provide a basis for breeding wheat varieties with enhanced phosphorus use efficiency.