Surface Disinfection and Sanitizing Action of the Alcohol-Free Essential Oil-based Green Formulation

The surface disinfectant property of a prepared formulation using potential and effective EO (Murraya koenigii), phytochemical (Geraniol), and an amino acid epsilon-L-Poly-Lysine (ɛ-PL) is examined in this present study. To investigate its potential as a surface disinfectant (SD) different tests using multiple bacterial strains were conducted. All tested bacterial strains were inhibited by the SD treatments, with a MIC range of (0.78-3.12%) v/v. Notably, Staphylococcus sp. was found to be more susceptible to the treatment than its gram-negative counterparts. In the test, sterile stainless-steel surfaces were used and externally contaminated with Escherichia sp. Cleaning the surface with the prepared formulation was more effective than the equal concentration of vinegar in terms of bacterial growth reduction. Vinegar was used as a mother solvent in the preparation of the SD due to its proven antibacterial effect. It is worth mentioning, this formulation is also proven to be effective on biofilm-embedded bacterial cells of Pseudomonas aeruginosa PAO1 as found in epifluorescence microscopy staining. Even though the impact of each constituent needs to be further explored, the effectiveness of this formulation may encourage large farms to seek out alternatives that are more environmentally friendly, safe, and effective than conventional products.