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Updated: Aug 4, 2025

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
Genome-Wide Identification of Variants Associated with Antifungal Drug Resistance
Aina Martinez-Zurita1, Christina A Cuomo2
1Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Abstract:
Genomic approaches are widely applied to study the genetic basis of antifungal drug resistance in clinical isolates and experimental studies. Whole-genome sequencing of clinical isolates can comprehensively identify mutations associated with drug resistance and their frequency across fungal populations. In addition, genome comparison of serially collected isolates, such as from patient samples or in vitro drug selection experiments, will identify a small number of changes that can be evaluated for association with drug resistance. Here, we provide a detailed protocol for the computational analysis of genome sequences to identify variants associated with drug resistance.
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