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Updated: Aug 4, 2025

08:45
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
13.8K
Characterizing Candida glabrata Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistance
Lucia Simonicova1, W Scott Moye-Rowley2
1Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 6, 2023
Abstract:
This chapter illustrates how to prepare isogenic strains carrying gain-of-function forms of transcription factor Pdr1 in the human pathogen Candida glabrata. Simple steps are described that lead from a characterized plasmid-borne PDR1-GOF allele to its integration into the yeast genome in a markerless manner. Pdr1-GOF strains constructed by this approach are suitable for virulence studies in an animal host.
Keywords:
Azole resistanceCandida glabrataIsogenic strainsMarker-less integration of PDR1-GOF into the genomeTranscription factor Pdr1
