Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardioprotection by <i>Allium ampeloprasum subsp. Iranicum</i>: attenuation of cyclophosphamide-induced cardiotoxicity via modulation of oxidative stress and inflammatory mediator.

Current research in physiology·2026
Same author

Engineering a synthetic niche for hADSC preconditioning with PRP, Lp-SN, and resveratrol nanoparticles.

Artificial cells, nanomedicine, and biotechnology·2026
Same author

The Assessment of the Effect of Nano Propolis Against Melanoma Cell Line, and Its Radio Sensitization Effect.

Food science & nutrition·2026
Same author

CD20-targeted immunotherapies in B-cell malignancies: mechanistic and clinical advances.

Experimental hematology·2026
Same author

Transforming Duchenne muscular dystrophy therapy: The multifaceted role of extracellular vesicles and exosomes.

Biochemistry and biophysics reports·2026
Same author

Development of AS1411 aptamer-conjugated chitosan-carbon dot nanocarriers for targeted drug delivery and fluorescent imaging in breast cancer therapy.

Journal of biological engineering·2026

Related Experiment Video

Updated: Aug 4, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

14.6K

An efficient method for cell sheet bioengineering from rBMSCs on thermo-responsive PCL-PEG-PCL copolymer.

Sevil Vaghefi Moghaddam1, Fatemeh Abedi1, Hajie Lotfi2

  • 1Clinical Research Development, Unit of Tabriz Valiasr Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.

Journal of Biological Engineering
|April 6, 2023
PubMed
Summary

Sodium selenite and vitamin C enhance rat bone marrow-derived mesenchymal stem cell (rBMSC) sheet quality by promoting stemness and reducing senescence. Sodium selenite supplementation yielded superior cell sheets compared to vitamin C alone.

Keywords:
Cell sheetsPCL-PEG-PCLSodium SeleniteSol-GelStem cell

More Related Videos

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
07:44

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

7.5K
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.4K

Related Experiment Videos

Last Updated: Aug 4, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

14.6K
Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
07:44

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

7.5K
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.4K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Cell sheet technology utilizes thermos-responsive substrates to maintain cell-cell junctions and extracellular matrix (ECM).
  • Rat bone marrow-derived mesenchymal stem cells (rBMSCs) are a promising source for regenerative medicine applications.
  • Optimizing cell sheet quality is crucial for effective therapeutic outcomes.

Purpose of the Study:

  • To investigate the efficacy of sodium selenite and a PCL-PEG-PCL copolymer in forming high-quality cell sheets from rBMSCs.
  • To evaluate the impact of different medium enrichments, including antioxidants, on rBMSC proliferation and cell sheet characteristics.
  • To analyze the molecular mechanisms underlying cell sheet formation and quality, focusing on stemness and senescence markers.

Main Methods:

  • Synthesis and characterization of the PCL-PEG-PCL (PCEC) thermos-responsive copolymer.
  • Culture of rBMSCs on PCEC substrates in media supplemented with vitamin C, sodium selenite, Trolox, or a routine medium.
  • Phenotypic and molecular analysis of harvested cell sheets, including gene expression (Sox2, Oct-4, Nanog, TRX, GPX-1, Sirt1) and senescence assays (beta-galactosidase).

Main Results:

  • PCEC copolymer exhibited sol-gel-sol phase transition properties suitable for cell sheet harvesting.
  • Supplementation with vitamin C, sodium selenite, and Trolox improved rBMSC osteogenesis and adipogenesis.
  • Cell sheets cultured with sodium selenite or vitamin C showed reduced senescence and enhanced expression of stemness genes.
  • Sodium selenite supplementation resulted in higher-quality cell sheets compared to vitamin C alone.

Conclusions:

  • Sodium selenite and vitamin C are effective in improving the quality of rBMSC-derived cell sheets.
  • The combination of thermos-responsive substrates and specific medium supplementation can enhance cell sheet integrity and therapeutic potential.
  • Sodium selenite shows particular promise for optimizing cell sheet technology in regenerative medicine.