Related Experiment Video
Updated: Aug 3, 2025
09:43
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
Published on: October 31, 2013
13.6K
Over One Million DNA and Protein Events Through Ultra-Stable Chemically-Tuned Solid-State Nanopores
Jugal Saharia1,2, Yapa Mudiyanselage Nuwan Dhananjaya Yapa Bandara3, Buddini Iroshika Karawdeniya4
1Department of Mechanical Engineering, Southern Methodist University, Dallas, TX, 75275, USA.
Small (Weinheim an Der Bergstrasse, Germany)
|April 7, 2023
Summary
This study introduces a novel fabrication protocol for solid-state nanopores, achieving over 8 million DNA and protein detection events. This breakthrough enhances nanopore performance for applications like machine learning.
Area of Science:
- Nanotechnology
- Biophysics
- Analytical Chemistry
Background:
- Solid-state nanopore technology is critical for various applications.
- Key performance metrics include stability, longevity, clog resistance, low noise, and cost-effectiveness.
Purpose of the Study:
- To develop and present a fabrication protocol for solid-state nanopores.
- To demonstrate unprecedented event counts and long-term operational stability.
- To showcase a real-time pore cleaning method.
Main Methods:
- Fabrication of solid-state nanopores.
- High-throughput event detection using DNA and proteins.
- Utilizing a 100 kHz lowpass filter (LPF) for signal acquisition.
- Real-time pore cleaning and revival techniques.
Main Results:
- Achieved >1 million events from a single nanopore and ≈8.1 million total events across DNA and protein analytes.
- Demonstrated sustained pore operation for >7 hours with minimal pore growth (≈0.16 nm/h).
- Observed exceptionally stable current noise (<10 pA/h increase) and a real-time cleaning method with <5% diameter change.
Conclusions:
- The developed fabrication protocol significantly advances solid-state nanopore performance.
- The high event counts and stability provide large, pristine datasets suitable for machine learning.
- The real-time cleaning method enhances nanopore reusability and operational lifetime.
