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The Biochemical Characteristics in Experimental Keratomycosis
1Department of Optometry and Ophthalmology, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, China.
Purpose:
To investigate the biochemical characteristics in experimental keratomycosis.
Methods:
Experimental mice were injected with Fusarium solanum solution Controls mice received liposomes containing phosphate-buffered saline (PBS-LIP). Raman spectroscopy was used to analyze the biochemical characteristics. The infiltration of inflammatory cells was analyzed by histopathology. Cytokine mRNA levels were detected by real-time polymerase chain reaction.
Results:
Raman Spectroscopy: In the experimental group, collagen, lipids, amide I and III were decreased, amide II, hyper proline amino acids, and arginine were increased, and proline and phenylalanine were significantly increased on day 3. Histopathology: more severe keratomycosis developed in the experimental group than in the control group. Statistically significant mRNA expression of Collagen4\MMP2\MMP9\TIMP1.MMP9 was negatively correlated with the secretion of Collagen4.
Conclusions:
Matrix metalloproteinases are involved in biochemical changes in keratomycosis.
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