The Biochemical Characteristics in Experimental Keratomycosis
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1Department of Optometry and Ophthalmology, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, China.
Purpose:
To investigate the biochemical characteristics in experimental keratomycosis.
Methods:
Experimental mice were injected with Fusarium solanum solution Controls mice received liposomes containing phosphate-buffered saline (PBS-LIP). Raman spectroscopy was used to analyze the biochemical characteristics. The infiltration of inflammatory cells was analyzed by histopathology. Cytokine mRNA levels were detected by real-time polymerase chain reaction.
Results:
Raman Spectroscopy: In the experimental group, collagen, lipids, amide I and III were decreased, amide II, hyper proline amino acids, and arginine were increased, and proline and phenylalanine were significantly increased on day 3. Histopathology: more severe keratomycosis developed in the experimental group than in the control group. Statistically significant mRNA expression of Collagen4\MMP2\MMP9\TIMP1.MMP9 was negatively correlated with the secretion of Collagen4.
Conclusions:
Matrix metalloproteinases are involved in biochemical changes in keratomycosis.
Insights
This study reveals significant biochemical alterations in experimental keratomycosis, highlighting the role of matrix metalloproteinases in disease progression. These findings offer insights into the molecular mechanisms of fungal eye infections.
Area of Science:
- Ophthalmology
- Biochemistry
- Microbiology
Background:
- Keratomycosis, a fungal infection of the cornea, presents a significant challenge in clinical practice.
- Understanding the biochemical changes associated with keratomycosis is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the biochemical characteristics of experimental keratomycosis.
- To elucidate the role of specific biomolecules and enzymes in fungal keratitis progression.
Main Methods:
- Induction of experimental keratomycosis in mice using *Fusarium solanum*.
- Analysis of biochemical composition using Raman spectroscopy.
- Histopathological examination for inflammatory cell infiltration.
- Quantification of cytokine mRNA levels via real-time polymerase chain reaction.
Main Results:
- Raman spectroscopy revealed decreased collagen and lipids, and increased proline, phenylalanine, and amide II in the experimental group.
- Histopathology confirmed more severe keratomycosis in the infected group compared to controls.
- Significant mRNA expression of Collagen4, MMP2, MMP9, and TIMP1 was observed, with MMP9 negatively correlated to Collagen4 secretion.
Conclusions:
- Matrix metalloproteinases (MMPs) play a critical role in the biochemical alterations observed during keratomycosis.
- The study provides a detailed biochemical profile of fungal keratitis, implicating MMPs in disease pathogenesis.
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