Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Internal Combustion Engine01:20

Internal Combustion Engine

1.5K
The internal combustion engine is a heat engine that uses the byproducts of combustion as the working fluid instead of using a heat transfer medium to transfer heat. The combustion is done in a way that produces high-pressure combustion products that can be expanded through a turbine or piston to create work. Internal combustion engines can again be categorized into three kinds: (1) spark ignition gasoline engines, most commonly used in automobiles, (2) compression ignition diesel engines that...
1.5K
Dynamics Of Circular Motion: Applications01:17

Dynamics Of Circular Motion: Applications

7.9K
Suppose a car moves on flat ground and turns to the left. The centripetal force causing the car to turn in a circular path is due to friction between the tires and the road. For this, a minimum coefficient of friction is needed, or the car will move in a larger-radius curve and leave the roadway. Let's now consider banked curves, where the slope of the road helps in negotiating the curve. The greater the angle of the curve, the faster one can take the curve. It is common for race tracks for...
7.9K
Drag Force and Terminal Speed01:18

Drag Force and Terminal Speed

2.4K
An interesting force in everyday life is the force of drag on an object when it is moving in a fluid. Like friction, the drag force always opposes the motion of an object. Unlike simple friction, the drag force is proportional to some function of the velocity of the object in that fluid. This functionality is complicated and depends upon the shape of the object, its size, its velocity, and the fluid it is in. For most large objects, such as cyclists, cars, and baseballs, that are not moving too...
2.4K
Torque01:10

Torque

15.5K
Torque is an important quantity for describing the dynamics of a rotating rigid body. We see the application of torque in many ways in the world, such as when pressing the accelerator in a car, which causes the engine to apply additional torque on the drivetrain. Here, we define torque and provide a framework to create an equation to calculate torque for a rigid body with fixed-axis rotation.
Torque can be considered as the rotational counterpart to force. Since forces change the translational...
15.5K
Rolling Resistance01:21

Rolling Resistance

333
When a solid cylinder rolls steadily on a rigid surface, the normal force applied by the surface on the cylinder is perpendicular to the tangent at the contact point. However, since no materials are entirely rigid, the surface's reaction to the cylinder involves a range of normal pressures.
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down...
333
Instantaneous Acceleration01:16

Instantaneous Acceleration

7.9K
Acceleration is in the direction of the change in velocity, but it is not always in the direction of motion. When an object slows down, its acceleration is opposite to the direction of its motion. Although commonly referred to as deceleration, this causes confusion in our analysis as deceleration is not a vector, and does not point to a specific direction with respect to a coordinate system. Therefore, the term deceleration is not used. For example, when a subway train slows down, it...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Let your light shine down.

Science immunology·2026
Same author

A novel hyperactive <i>BCR::ABL1</i> <sup><i>e6a3</i></sup> variant confers resistance to combined asciminib plus ponatinib therapy.

medRxiv : the preprint server for health sciences·2026
Same author

The Clinicopathologic and Genomic Features of Mature Versus Blastic Plasmacytoid Dendritic Cell Neoplasms Arising From Chronic Myeloid Neoplasms.

The American journal of surgical pathology·2026
Same author

Blastic Plasmacytoid Dendritic Cell Neoplasm: Updates in Diagnostic and Molecular Pathology.

Surgical pathology clinics·2026
Same author

Structural variation tunes apoptotic responses to drive immune escape in melanoma.

Science immunology·2026
Same author

Early persistence of recipient stem-cells and T-cell dysregulation are associated with relapse after transplant in AML/MDS.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Aug 3, 2025

A Rapid Method for Modeling a Variable Cycle Engine
04:58

A Rapid Method for Modeling a Variable Cycle Engine

Published on: August 13, 2019

7.6K

You've got a fast CAR.

Ángel Garza Reyna1, Gabriel K Griffin1,2

  • 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Science Immunology
|April 7, 2023
PubMed
Summary

Disrupting TET2 enhances chimeric antigen receptor T-cell (CAR-T) therapy effectiveness. However, this genetic modification introduces potential risks and limitations that require careful consideration for clinical application.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cancer Research

Background:

  • Chimeric antigen receptor T-cell (CAR-T) therapy is a promising cancer treatment.
  • Enhancing CAR-T cell efficacy remains a critical area of research.
  • The role of epigenetic regulators in CAR-T cell function is increasingly recognized.

Purpose of the Study:

  • To investigate the impact of TET2 disruption on CAR-T cell function.
  • To evaluate the potential benefits and drawbacks of TET2-modified CAR-T cells.

Main Methods:

  • Genetic modification of T-cells to disrupt the TET2 gene.
  • In vitro and in vivo assays to assess CAR-T cell proliferation, cytotoxicity, and persistence.
  • Analysis of epigenetic modifications in TET2-disrupted CAR-T cells.

More Related Videos

Implementation of Portable Emissions Measurement Systems PEMS for the Real-driving Emissions RDE Regulation in Europe
09:34

Implementation of Portable Emissions Measurement Systems PEMS for the Real-driving Emissions RDE Regulation in Europe

Published on: December 4, 2016

28.2K
The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis
11:43

The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis

Published on: February 5, 2014

27.4K

Related Experiment Videos

Last Updated: Aug 3, 2025

A Rapid Method for Modeling a Variable Cycle Engine
04:58

A Rapid Method for Modeling a Variable Cycle Engine

Published on: August 13, 2019

7.6K
Implementation of Portable Emissions Measurement Systems PEMS for the Real-driving Emissions RDE Regulation in Europe
09:34

Implementation of Portable Emissions Measurement Systems PEMS for the Real-driving Emissions RDE Regulation in Europe

Published on: December 4, 2016

28.2K
The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis
11:43

The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis

Published on: February 5, 2014

27.4K

Main Results:

  • TET2 disruption significantly improved CAR-T cell anti-tumor activity.
  • Enhanced CAR-T cell persistence and reduced exhaustion markers were observed.
  • Potential off-target effects and metabolic alterations were identified as consequences of TET2 disruption.

Conclusions:

  • TET2 disruption represents a viable strategy for augmenting CAR-T cell therapy.
  • The benefits of TET2 modification must be weighed against associated risks.
  • Further research is warranted to optimize TET2-based CAR-T cell engineering for safe and effective clinical translation.