Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Adult Stem Cells01:33

Adult Stem Cells

30.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
30.4K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

3.7K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Characterization of Double-Bond Reductases in Dihydro-β-Ionone Biosynthesis in <i>Cymbidium sinense</i>.

Plants (Basel, Switzerland)·2025
Same author

Targeting NRP1 in Endothelial Cells Facilitates the Normalization of Scar Vessels and Prevents Fibrotic Scarring.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Evaluating Rectus Abdominis Fascia and Alloderm as Wrapping Materials in Diced Cartilage Grafts for Dorsal Nasal Augmentation.

Aesthetic plastic surgery·2025
Same author

Positional BMP signaling orchestrates villus length in the small intestine.

Nature communications·2025
Same author

YTHDF2-KIF26B-Wnt signaling forms a positive-feedback regulatory loop to maintain intestinal stem cell stemness.

Cell regeneration (London, England)·2025
Same author

Mitsugumin 53 drives stem cell differentiation easing intestinal injury and inflammation.

Signal transduction and targeted therapy·2025

Related Experiment Video

Updated: Aug 3, 2025

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.3K

Segregation of the stemness program from the proliferation program in intestinal stem cells.

Yuan Liu1, Meimei Huang1, Xiaodan Wang1

  • 1The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.

Stem Cell Reports
|April 7, 2023
PubMed
Summary

Methyltransferase-like 3 (Mettl3) enzyme maintains intestinal stem cell (ISC) stemness via m6A methylation. This crucial function is separable from cell proliferation, revealing a new mechanism for stemness regulation.

Keywords:
Lgr5Mettl3intestinal stem cellsm6A modificationproliferationstemness

More Related Videos

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
06:40

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

4.9K
Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
10:57

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Published on: December 16, 2014

20.5K

Related Experiment Videos

Last Updated: Aug 3, 2025

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.3K
3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
06:40

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

4.9K
Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
10:57

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Published on: December 16, 2014

20.5K

Area of Science:

  • Stem cell biology
  • Epigenetics
  • Gastroenterology

Background:

  • Intestinal stem cells (ISCs) are vital for gut homeostasis, possessing self-renewal and differentiation capabilities.
  • The relationship between stem cell proliferation and stemness maintenance remains incompletely understood.
  • N6-methyladenosine (m6A) methylation is an important epitranscriptomic modification involved in various cellular processes.

Purpose of the Study:

  • To investigate the role of methyltransferase-like 3 (Mettl3) and m6A methylation in maintaining the stemness of Lgr5+ intestinal stem cells (ISCs).
  • To determine if the proliferative capacity of ISCs can be functionally uncoupled from their stemness properties.
  • To identify key molecular players regulated by m6A methylation that sustain ISC stemness.

Main Methods:

  • CRISPR-Cas9 mediated gene deletion of Mettl3 in ISCs.
  • Analysis of stemness and proliferation markers in Mettl3-deficient intestinal organoids.
  • Ectopic expression and silencing of identified m6A-modified transcriptional factors.
  • Transcriptomic profiling (RNA-sequencing) to identify differentially expressed genes.

Main Results:

  • Mettl3 deletion in ISCs led to rapid loss of stemness markers without affecting cell proliferation.
  • Ectopic expression of four identified m6A-modified transcription factors rescued stemness gene expression in Mettl3-deficient organoids.
  • Silencing of these transcription factors resulted in loss of stemness.
  • Transcriptomic analysis identified 23 genes associated with stemness that are distinct from proliferation-related genes.

Conclusions:

  • Mettl3-mediated m6A methylation is essential for sustaining ISC stemness.
  • ISC stemness can be functionally segregated from cell proliferation.
  • m6A modification regulates specific transcriptional factors that maintain stemness, independent of proliferation control.