Tooth enamel nitrogen isotope composition records trophic position: a tool for reconstructing food webs

Nitrogen isotopes are widely used to study the trophic position of animals in modern food webs; however, their application in the fossil record is severely limited by degradation of organic material during fossilization. In this study, we show that the nitrogen isotope composition of organic matter preserved in mammalian tooth enamel (δ¹⁵N_enamel) records diet and trophic position. The δ¹⁵N_enamel of modern African mammals shows a 3.7‰ increase between herbivores and carnivores as expected from trophic enrichment, and there is a strong positive correlation between δ¹⁵N_enamel and δ¹⁵N_{bone-collagen} values from the same individuals. Additionally, δ¹⁵N_enamel values of Late Pleistocene fossil teeth preserve diet and trophic level information, despite complete diagenetic loss of collagen in the same specimens. We demonstrate that δ¹⁵N_enamel represents a powerful geochemical proxy for diet that is applicable to fossils and can help delineate major dietary transitions in ancient vertebrate lineages.