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Updated: Aug 3, 2025
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Published on: September 30, 2010
Prophylactic antineoplastic activity of Toxoplasma gondii RH derived antigen against ehrlich solid carcinoma with
Maha M Eissa1, Maha R Gaafar1, Layla K Younis2
1Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Background:
With cancer cases escalation, an urgent request to develop novel combating strategies arise. Pathogen-based cancer-immunotherapy is getting more consideration. Autoclaved parasitic antigens seem promising candidates, taking steadily their first steps. Our aim was to examine the prophylactic antineoplastic activity of autoclaved Toxoplasma vaccine (ATV) and to test for the shared antigen theory between Toxoplasma gondii and cancer cells.
Methods:
Mice were immunized with ATV followed by Ehrlich solid carcinoma (ESC) inoculation. Tumor weight, volume, histopathology, and immunohistochemistry for CD8+ T cells, Treg cells and VEGF were assessed. In addition, the proposed shared antigen theory between parasites and cancer was also verified using SDS-PAGE and immunoblotting.
Results:
Results revealed powerful prophylactic activity of ATV with 13.3% inhibition of ESC incidence, significant reduction in tumor weight and volume in ATV vaccinated mice. Immunologically, significantly higher CD8+T cells and lower FOXP3+ Treg cells surrounded and infiltrated ESC in ATV immunized mice with higher CD8+T/Treg cells ratio and significant antiangiogenic effect. Moreover, SDS-PAGE and immunoblotting showed four shared bands between Ehrlich carcinoma and ATV of approximate molecular weights 60, 26, 22 and 12.5 KDa.
Conclusion:
Exclusively, we demonstrated a prophylactic antineoplastic activity of autoclaved Toxoplasma vaccine against ESC. Moreover, to the best of our knowledge this is the first report highlighting the existence of cross-reactive antigens between Toxoplasma gondi parasite and cancer cells of Ehrlich carcinoma.
Insights
Autoclaved Toxoplasma vaccine (ATV) demonstrated prophylactic antineoplastic effects against Ehrlich solid carcinoma (ESC) in mice. This study also identified shared antigens between Toxoplasma gondii and ESC, supporting cross-reactive immunotherapy potential.
Area of Science:
- Immunology
- Oncology
- Parasitology
Background:
- Rising cancer incidence necessitates novel therapeutic strategies.
- Pathogen-based cancer immunotherapy is an emerging field.
- Autoclaved parasitic antigens show potential for cancer treatment.
Purpose of the Study:
- To evaluate the prophylactic antineoplastic activity of autoclaved Toxoplasma vaccine (ATV).
- To investigate shared antigens between Toxoplasma gondii and cancer cells.
Main Methods:
- Mice were immunized with ATV and inoculated with Ehrlich solid carcinoma (ESC).
- Tumor characteristics, immune cell infiltration (CD8+, Treg), and VEGF were analyzed.
- SDS-PAGE and immunoblotting were used to identify shared antigens.
Main Results:
- ATV significantly inhibited ESC incidence and reduced tumor weight/volume.
- ATV vaccination increased CD8+ T cells and decreased Treg cells, enhancing the CD8+/Treg ratio.
- Four shared antigen bands (60, 26, 22, 12.5 KDa) were detected between ATV and ESC.
Conclusions:
- ATV exhibits significant prophylactic antineoplastic activity against ESC.
- This study provides the first evidence of cross-reactive antigens between Toxoplasma gondii and Ehrlich carcinoma.
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