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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Aug 3, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Recent progress in aptamer and CRISPR-Cas12a based systems for non-nucleic target detection.

Ruiqi Yang1,2, Liping Zhao1, Xinjie Wang1

  • 1Agricultural Product Quality and Safety Risk Assessment Laboratory of the Department of Agriculture, Institute of Quality Standard and Testing Technology of BAAFS, Beijing 100097, China.

Critical Reviews in Analytical Chemistry
|April 8, 2023
PubMed
Summary

CRISPR-Cas12a and aptamers offer powerful biosensor capabilities for detecting non-nucleic acid targets. This review summarizes their progress, challenges, and design considerations for improved sensitivity and diverse applications.

Keywords:
AptamerCRISPR-Cas12abiosensornon-nucleic acid target

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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Area of Science:

  • Biotechnology and Biosensor Development
  • Molecular Biology and Gene Editing Tools

Background:

  • Biosensors are crucial for sensitive target detection, driving innovation in sensor technology.
  • CRISPR-Cas12a, a gene editing tool, shows significant promise for biosensor design.
  • Aptamers, selected via SELEX, serve as recognition elements for CRISPR-Cas12a in biosensors.

Purpose of the Study:

  • To review the working principles of CRISPR-Cas12a/Apt biosensors for non-nucleic acid targets.
  • To summarize recent advancements in CRISPR-Cas12a/Apt applications for detecting diverse targets.
  • To discuss critical design parameters for developing novel, efficient, and sensitive biosensors.

Main Methods:

  • Review of literature on CRISPR-Cas12a/Apt biosensor technology.
  • Analysis of CRISPR-Cas12a's collateral activity with aptamer recognition.
  • Discussion of key parameters: crRNA sequence, activator sequence, and reaction system.

Main Results:

  • CRISPR-Cas12a/Apt biosensors demonstrate potential for detecting small molecules, proteins, viruses, and bacteria.
  • Current limitations include single signal modes and narrow linear ranges in existing biosensors.
  • Optimization of design parameters can enhance biosensor performance for various detection scenarios.

Conclusions:

  • CRISPR-Cas12a/Apt technology offers a versatile platform for non-nucleic acid biosensing.
  • Addressing current limitations is essential for broader adoption and improved detection capabilities.
  • Further research into diverse CRISPR-Cas12a/Apt biosensor designs is necessary for future applications.