Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Acta crystallographica. Section D, Structural biology·2026
Same author

Rational acquisition of laboratory equipment: an accurate mathematical model to estimate the trade-offs in shared and nonshared equipment.

Acta crystallographica. Section D, Structural biology·2026
Same author

Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and influences the formation of reovirus factories.

Journal of virology·2025
Same author

How many (distinguishable) classes can we identify in single-particle analysis?

Acta crystallographica. Section D, Structural biology·2025
Same author

Automatic detection of alignment errors in cryo-electron tomography.

Journal of structural biology·2024
Same author

Real-space heterogeneous reconstruction, refinement, and disentanglement of CryoEM conformational states with HetSIREN.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Aug 3, 2025

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

50.4K

ZART: A Novel Multiresolution Reconstruction Algorithm with Motion-blur Correction for Single Particle Analysis.

D Herreros1, J Kiska2, E Ramírez-Aportela3

  • 1Centro Nacional de Biotecnologia-CSIC, C/ Darwin, 3, 28049, Cantoblanco, Madrid, Spain. Electronic address: https://twitter.com/HerosCGM.

Journal of Molecular Biology
|April 8, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces ZART, a new method for Cryo-Electron Microscopy (CryoEM) single particle analysis. ZART reconstructs macromolecule structures by accounting for flexibility, reducing motion blurring and improving resolution in 3D maps.

Keywords:
Cryo-Electron Microscopy (CryoEM)Zernike polynomialsZernike3D-based Algebraic Reconstruction Technique (ZART)map reconstructionspherical harmonics

More Related Videos

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

11.5K
Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
07:38

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Published on: April 9, 2017

10.2K

Related Experiment Videos

Last Updated: Aug 3, 2025

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

50.4K
High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

11.5K
Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
07:38

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Published on: April 9, 2017

10.2K

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Cryo-Electron Microscopy Single Particle Analysis (CryoEM SPA) aims to determine 3D macromolecule structures from 2D images.
  • Standard 3D reconstruction methods assume static molecules, leading to artifacts in flexible specimens due to motion blurring.

Purpose of the Study:

  • Introduce Zernike Algebraic Reconstruction Technique (ZART) to address limitations in CryoEM SPA for flexible macromolecules.
  • Improve local resolution and reduce motion blurring in 3D CryoEM maps by incorporating continuous flexibility information.

Main Methods:

  • Developed ZART, an Algebraic Reconstruction Technique incorporating continuous flexibility.
  • Modeled conformational changes using Zernike3D polynomials.
  • Implemented a multiresolution approach adapting to local map resolution.

Main Results:

  • ZART effectively reduces motion-induced artifacts in CryoEM maps of flexible specimens.
  • The algorithm improves both local and global resolution of reconstructed 3D volumes.
  • ZART enhances map quality even for less flexible macromolecules.

Conclusions:

  • ZART offers a significant advancement for CryoEM SPA, particularly for flexible biological samples.
  • The method provides higher resolution and clearer structural details compared to conventional techniques.
  • ZART is a versatile tool for improving the quality of CryoEM reconstructions across various sample types.