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Updated: Aug 3, 2025

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

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Published on: January 21, 2020

First continuous marine sponge cell line established.

Kylie Hesp¹, Jans M E van der Heijden², Stephanie Munroe^2,3

¹Bioprocess Engineering, Wageningen University and Research, Wageningen, The Netherlands. kylie.hesp@gmail.com.

Scientific Reports

|April 8, 2023

Summary

This summary is machine-generated.

Researchers established the first continuous marine sponge cell line from Geodia barretti. This breakthrough enables scalable production of valuable sponge-derived biopharmaceuticals for industrial applications.

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Area of Science:

Marine Biology
Biotechnology
Drug Discovery

Background:

Marine sponges are a rich source of novel compounds with pharmaceutical potential.
Limited biomass from natural sponge populations hinders the scalable production of these compounds.
Previous attempts to culture marine sponge cells in vitro for biopharmaceutical production have largely failed.

Purpose of the Study:

To establish the first continuous marine sponge cell line for scalable biopharmaceutical production.
To optimize culture conditions for rapid and high-density growth of Geodia barretti cells.
To overcome a significant barrier in utilizing sponge-derived compounds for industrial applications.

Main Methods:

Culturing Geodia barretti sponge cells in a modified M1 medium (OpM1).
Assessing cell growth rates and maximum population doublings in M1 versus OpM1.
Evaluating the cryopreservation and subculturing capabilities of the established cell line.

Main Results:

Established the first continuous marine sponge cell line from Geodia barretti.
Cells cultured in OpM1 exhibited significantly faster growth rates (1.74 doublings/30 min) compared to M1 (0.74 doublings/30 min).
OpM1 medium supported a substantial increase in maximum population doublings (≥98) compared to M1 (5).
The cell line demonstrated successful cryopreservation and subculturing.

Conclusions:

The development of a continuous Geodia barretti cell line in OpM1 medium overcomes a critical bottleneck for industrial-scale biopharmaceutical production.
This advancement paves the way for the sustainable and large-scale supply of potentially life-saving sponge-derived compounds.
The established cell line offers a robust platform for future research and development in marine natural product drug discovery.