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Updated: Aug 3, 2025

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Authentication of beef cuts by multielement and machine learning approaches
Yuniel Tejeda Mazola1, Elisabete A De Nadai Fernandes1, Gabriel A Sarriés2
1Nuclear Energy Center for Agriculture, University of São Paulo, Avenida Centenário 303, 13416-000 Piracicaba, SP, Brazil.
Background:
Brazil has consolidated a relevant position in the world market, being the largest exporter and second producer of beef. Genetics, feeding system, geographic origin and climate influence the multielement profile of beef. The feasibility of combining classification algorithms with major and trace elements was evaluated as a tool for authentication of beef cuts.
Methods:
Animals of Angus, Nelore and Wagyu crossbreeds, raised in a vertically integrated system, were sampled at the slaughterhouse for chuck steak, rump cap and sirloin steak. Supervised learning algorithms i.e. Classification and Regression Tree (CART), Multilayer Perceptron (MLP), Naïve Bayes (NB), Random Forest (RF) and Sequential Minimal Optimization (SMO) were used to build classification models based on the multielement profile of beef determined by neutron activation analysis.
Results:
Br, Co, Cs, Fe, K, Na, Rb, Se and Zn were determined in the beef samples. The classification accuracy values obtained for the beef cuts were 96% (MLP), 95% (SMO), 91% (RF), 86% (NB) and 70% (CART).
Conclusion:
The Multilayer Perceptron algorithm provided the best classification performance towards authentication of beef cuts on basis of major and trace element mass fractions.
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