Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine learning ensemble reveals distinct molecular pathways of retinal damage in spaceflown mice.

NPJ microgravity·2026
Same author

Proximal Junctional Kyphosis Prevention in Adult Spinal Deformity Surgery: A Technical Review of Tethering and Adjunctive Strategies.

Operative neurosurgery (Hagerstown, Md.)·2026
Same author

Integrative genomic profiling of iron homeostasis predicts clinical outcomes and identifies HAMP/hepcidin as a therapeutic target in colorectal cancer.

Cell & bioscience·2026
Same author

Principles of gene therapy of the inner ear.

Current opinion in otolaryngology & head and neck surgery·2025
Same author

Human inner ear fluid imbalance detected by optical coherence tomography correlates with hearing loss.

Science translational medicine·2025
Same author

Facial Nerve Amyloidosis Leading to Unilateral Facial Paralysis and Sensorineural Hearing Loss.

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·2025

Related Experiment Video

Updated: Aug 3, 2025

Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice
09:06

Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice

Published on: January 9, 2019

14.0K

Neomycin-induced deafness in neonatal mice.

Raffaello M Cutri1, Joshua Lin1, Nhi V Nguyen2

  • 1Keck School of Medicine of University of Southern California, Los Angeles, CA 90033, USA.

Journal of Neuroscience Methods
|April 9, 2023
PubMed
Summary
This summary is machine-generated.

A single neomycin injection into the neonatal mouse inner ear rapidly causes hair cell loss and nerve damage, creating a reliable model for studying deafness and auditory development.

Keywords:
AminoglycosidesHair cellsHearing lossNeomycinSpiral ganglion cells

More Related Videos

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research
08:30

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Published on: June 8, 2017

17.8K
Author Spotlight: Advancements in Cultivating Mouse Hair Cells for Auditory Research
06:07

Author Spotlight: Advancements in Cultivating Mouse Hair Cells for Auditory Research

Published on: September 15, 2023

2.5K

Related Experiment Videos

Last Updated: Aug 3, 2025

Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice
09:06

Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice

Published on: January 9, 2019

14.0K
Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research
08:30

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Published on: June 8, 2017

17.8K
Author Spotlight: Advancements in Cultivating Mouse Hair Cells for Auditory Research
06:07

Author Spotlight: Advancements in Cultivating Mouse Hair Cells for Auditory Research

Published on: September 15, 2023

2.5K

Area of Science:

  • Oto-neurology
  • Developmental biology
  • Pharmacology

Background:

  • Hearing impairment is a growing public health concern with limited therapeutic options.
  • Animal models are crucial for understanding deafness and developing hearing restoration strategies.

Purpose of the Study:

  • To establish a reliable and rapid deafening model in neonatal mice.
  • To investigate the effects of inner ear damage during auditory maturation.

Main Methods:

  • Neonatal mice (P2-5) received micro-injections of neomycin into the endolymphatic space.
  • Cochlear morphology, hair cell (HC) loss, supporting cell changes, and neurite denervation were assessed.
  • Spiral ganglion neuron (SGN) density and auditory brain response (ABR) were measured post-injection.

Main Results:

  • Neomycin induced rapid and complete degeneration of inner and outer HCs within 3 days.
  • Significant SGN density reduction and profound deafness (confirmed by ABR) were observed at 4 weeks.
  • Time- and dose-dependent degeneration patterns were noted along the cochlear axis.

Conclusions:

  • A single endolymphatic neomycin injection reliably induces significant hair cell loss and denervation in neonatal mice.
  • This method offers a robust and rapid deafening model for auditory research.
  • The model facilitates the study of inner ear damage during critical auditory development periods.