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Comparative genomic analysis of two ST320 Streptococcus pneumoniae isolates, representing serotypes 19A and 19F
Weronika Puzia1,2, Jan Gawor2, Robert Gromadka2
1Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland.
Background:
Streptococcus pneumoniae (pneumococcus) represents an important human pathogen, responsible for respiratory and invasive infections in the community. The efficacy of polysaccharide conjugate vaccines formulated against pneumococci is reduced by the phenomenon of serotype replacement in population of this pathogen. The aim of the current study was to obtain and compare complete genomic sequences of two pneumococcal isolates, both belonging to ST320 but differing by the serotype.
Results:
Here, we report genomic sequences of two isolates of important human pathogen, S. pneumoniae. Genomic sequencing resulted in complete sequences of chromosomes of both isolates, 2,069,241 bp and 2,103,144 bp in size, and confirmed the presence of cps loci specific for serotypes 19A and 19F. The comparative analysis of these genomes revealed several instances of recombination, which involved not only S. pneumoniae but also presumably other streptococci as donors.
Conclusions:
We report the complete genomic sequences of two S. pneumoniae isolates of ST320 and serotypes 19A and 19F. The detailed comparative analysis of these genomes revealed the history of several recombination events, clustered in the region including the cps locus.
Insights
Complete genome sequencing of two Streptococcus pneumoniae isolates revealed serotype differences due to recombination events. This finding is crucial for understanding pneumococcal evolution and vaccine efficacy.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Streptococcus pneumoniae (pneumococcus) is a significant human pathogen causing respiratory and invasive infections.
- Serotype replacement in pneumococcal populations reduces the effectiveness of polysaccharide conjugate vaccines.
- Understanding pneumococcal genetic diversity is key to combating infections.
Purpose of the Study:
- To obtain and compare complete genomic sequences of two pneumococcal isolates.
- To investigate genetic differences between isolates of the same sequence type (ST320) but different serotypes.
- To elucidate the role of recombination in pneumococcal serotype variation.
Main Methods:
- Whole-genome sequencing of two S. pneumoniae isolates.
- Comparative genomic analysis of the obtained sequences.
- Identification of recombination events and their locations.
Main Results:
- Complete chromosomal sequences were obtained for two S. pneumoniae isolates (2,069,241 bp and 2,103,144 bp).
- The isolates, both ST320, possessed cps loci corresponding to serotypes 19A and 19F.
- Comparative analysis identified multiple recombination instances, potentially involving other streptococci.
Conclusions:
- Complete genomic sequences of two S. pneumoniae isolates (ST320, serotypes 19A and 19F) were reported.
- Detailed comparative analysis revealed a history of recombination events.
- Recombination events were notably clustered around the cps locus, explaining serotype differences.
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