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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Aug 3, 2025

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Nanopore-based disease diagnosis using pathogen-derived tryptic peptides from serum.

Wenshu Zheng^1,2, Julian G Saliba^1,3, Xiaojun Wei^4,5

¹Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.

|April 10, 2023

Summary

This study presents a new method for selecting peptide biomarkers for nanopore sensors. The developed assay can detect tuberculosis biomarkers in serum, distinguishing between patients and controls with high accuracy.

Keywords:

Blood tests Infectious diseases Nanopore Peptide biomarkers

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Last Updated: Aug 3, 2025

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

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Published on: July 16, 2018

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

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Published on: April 17, 2012

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

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Published on: August 7, 2014

Area of Science:

Biomarker Discovery
Nanopore Sensing Technology
Clinical Diagnostics

Background:

Nanopore sensors are effective for nucleic acid analysis.
Peptide-nanopore interactions show potential for distinguishing peptide mixtures.
Clinical application of nanopore sensors is limited by specimen complexity.

Purpose of the Study:

To develop a methodology for selecting peptide biomarkers for clinical nanopore applications.
To adapt nanopore sensors for diagnosing diseases using peptide biomarkers.
To establish an immunoprecipitation-coupled nanopore (IP-NP) assay for clinical use.

Main Methods:

Developed criteria for selecting peptide biomarkers: pathogen specificity, antigenicity, charge, and solubility.
Utilized immunoprecipitation coupled with nanopore (IP-NP) assay.
Tested the assay using tuberculosis (TB) as a model disease.

Main Results:

Identified a TB-specific peptide biomarker.
The selected peptide generated high-affinity antibodies and a distinct nanopore signature.
The IP-NP assay detected and quantified the TB peptide in human serum with high sensitivity and specificity.
Differentiated between TB cases, healthy controls, and treated TB patients based on serum samples.

Conclusions:

The developed methodology enables selection of suitable peptide biomarkers for IP-NP assays.
The IP-NP assay demonstrates high sensitivity and specificity for detecting TB biomarkers in clinical samples.
This approach is adaptable for diagnosing other infectious and chronic diseases via peptide biomarkers.