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Placental Cell Conditioned Media Modifies Hematopoietic Stem Cell Transcriptome In Vitro.
Sean M Harris1, Anthony L Su2, John F Dou3
1School of Public Health, Department of Environmental Health Sciences, University of Michigan, 1415 Washington Heights, Ann Arbor, MI, 48109, USA.
Biorxiv : the Preprint Server for Biology
|April 10, 2023
Summary
Placental cells influence hematopoietic stem cells, affecting gene expression related to stem cell maintenance and proliferation. Exposure to monoethylhexyl phthalate (MEHP) alters specific genes involved in lipid metabolism.
Area of Science:
- Reproductive biology
- Hematology
- Toxicology
Background:
- Cross-talk between placental cells and hematopoietic stem cells is poorly understood.
- Impacts of toxicants on placental-hematopoietic stem cell communication are understudied.
- This study investigates placental factors' effects on hematopoietic stem cell transcriptomics.
Approach:
- Used K-562 cells (hematopoietic stem cell model) and BeWo cells (placental syncytiotrophoblast model) in vitro.
- Treated K-562 cells with BeWo-conditioned medium, with or without MEHP exposure.
- Performed RNA sequencing and differential gene expression analysis.
Key Points:
- BeWo-conditioned medium altered 173 genes in K-562 cells, impacting stem cell maintenance and proliferation pathways.
- Upregulated genes included TPM4 and S1PR3.
- MEHP treatment altered 8 genes, including those in lipid metabolism (PLIN2, CPT1A).
Conclusions:
- Hematopoietic stem cells modeled by K-562 cells respond to placental cell secretions.
- Placental factors may influence hematopoietic stem cell maintenance and proliferation pathways.
- Further research is needed on toxicant-mediated alterations in this communication.
