Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anticholinergic Medication Use in Veterans Affairs Long-Term Care Residents: Clinical Patterns and Opportunities for Deprescribing.

Journal of the American Geriatrics Society·2026
Same author

Interleukin-6 is associated with white matter hyperintensities and cognition independent of Alzheimer's disease plasma biomarkers-HABS-HD.

Journal of Alzheimer's disease : JAD·2026
Same author

Associations of plasma p-Tau217 with cognitive domain performance in clinically unimpaired participants: Evidence from HABS-HD.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026
Same author

Lead Lα<sub>1</sub> High Energy Resolution Fluorescence Detected X-ray Absorption Spectroscopy: A Powerful Tool for Chemical Speciation of Lead.

Inorganic chemistry·2026
Same author

White matter hyperintensities mediate a greater proportion of age-related lower cognitive scores in non-Hispanic White participants compared to non-Hispanic Black and Hispanic participants: HABS-HD.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026
Same author

Cu(I) and Cu(II) binding by hyperactive variants of the human CTR1 N-terminus: Insights from cellular and model peptide studies.

Journal of inorganic biochemistry·2026

Related Experiment Video

Updated: Aug 3, 2025

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
11:36

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

9.5K

Neuronal and Astrocyte Insulin-like Growth Factor-1 Signaling Differentially Modulates Ischemic Stroke Damage.

Cellas A Hayes1, Nyah I Morgan1, Kamryn C Thomas1

  • 1Department of BioMolecular Sciences, University of Mississippi School of Pharmacy, University of Mississippi, University, MS 386671.

Biorxiv : the Preprint Server for Biology
|April 10, 2023
PubMed
Summary

Insulin-like growth factor-1 (IGF-1) signaling in neurons and astrocytes impacts ischemic stroke damage. However, neither cell type individually requires IGF-1 signaling to minimize tissue damage or improve behavioral outcomes.

Keywords:
IGF-1Somatomedin Castrocytesglia-neuron interactionneuroprotectionneurovascular unitstroke

More Related Videos

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
15:08

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Published on: October 21, 2017

10.0K
AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke
05:40

AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke

Published on: November 29, 2024

541

Related Experiment Videos

Last Updated: Aug 3, 2025

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
11:36

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

9.5K
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
15:08

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

Published on: October 21, 2017

10.0K
AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke
05:40

AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke

Published on: November 29, 2024

541

Area of Science:

  • Neuroscience
  • Endocrinology
  • Vascular Biology

Background:

  • Ischemic stroke causes significant death and disability, with limited therapeutic options for long-term deficits.
  • Insulin-like growth factor-1 (IGF-1) shows promise in preclinical stroke models and clinical studies for reducing stroke risk and improving outcomes.
  • The precise cellular mechanisms of IGF-1 neuroprotection remain unclear, despite IGF-1 receptor expression across the neurovascular unit.

Approach:

  • Utilized inducible astrocyte-specific or neuron-specific transgenic mouse models to selectively reduce IGF-1 receptor (IGF-1R) in adult mice.
  • Administered photothrombotic stroke and assessed acute changes in blood-brain barrier permeability, microglial activation, and systemic inflammation.
  • Evaluated tissue damage and sensorimotor dysfunction for 3 days post-stroke, with and without IGF-1 supplementation.

Key Points:

  • Significant protection was observed in mice lacking neuronal and astrocytic IGF-1R immediately following stroke.
  • Neurological deficit scores improved over 3 days, with enhanced improvement upon IGF-1 supplementation.
  • Neither neuronal nor astrocytic IGF-1R deficiency alone was required for minimizing tissue damage or improving behavioral outcomes.

Conclusions:

  • Neuronal and astrocytic IGF-1 signaling plays a role in modulating stroke damage.
  • Individual cell-type specific IGF-1 signaling is not essential for mitigating acute tissue damage or long-term behavioral recovery.
  • Further research is needed to fully elucidate the complex role of IGF-1 in stroke pathophysiology and recovery.