Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Design Example: Capacitance Multiplier Circuit

Design Example: Capacitance Multiplier Circuit

In integrated circuit technology, a capacitance multiplier is often utilized to produce a larger capacitance value when a small physical capacitance falls short. This is achieved by a circuit that multiplies capacitance values by a factor of up to 1000, such that a 10-pF capacitor can replicate the performance of a 100-nF capacitor.
The circuit illustrated in Figure 1 below incorporates two op-amps, with the first operating as a voltage follower and the second acting as an inverting amplifier.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Lancet Commission on Sepsis: transforming sepsis care and outcomes.

Lancet (London, England)·2026

Same author

Occurrence and persistence of carbapenem-resistant Klebsiella pneumoniae complex in urban rivers of the São Paulo metropolitan region.

Journal of applied microbiology·2026

Same author

"Everyone in this together": a qualitative study on mothers as partners in infection prevention in a neonatal unit in Botswana.

Antimicrobial resistance and infection control·2026

Same author

Lyophilised colourimetric LAMP for visual readout with dual colour indicators.

The Analyst·2026

Same author

DYNAMIC: A Novel Software Implementation of a Kinetic Model of TaqMan PCR.

Analytical chemistry·2026

Same author

Early individualized risk prediction using clinical data for children during the febrile phase of dengue in outpatient settings in Vietnam and Thailand.

PLOS digital health·2026

Same journal

Translational opportunities in aptamer and nanobody lateral flow assays within the WHO REASSURED framework.

Sensors & diagnostics·2026

Same journal

Comparison of voltammetric methods used in the interrogation of electrochemical aptamer-based sensors.

Sensors & diagnostics·2026

Same journal

Biophysical characterization of <i>sp</i>Cas9 binding and cleavage using real-time electronic biosensors.

Sensors & diagnostics·2026

Same journal

A new sequential dual flow lab-on-a-chip with a lyophilized one-component chemiluminescence substrate for high-sensitive microchannel lateral flow assay (mLFA).

Sensors & diagnostics·2026

Same journal

Correction: High resolution voltammetric and field-effect transistor readout of carbon fiber microelectrode biosensors.

Sensors & diagnostics·2025

Same journal

Correction: Turn-on fluorescent sensors for Cu-rich amyloid β peptide aggregates.

Sensors & diagnostics·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 3, 2025

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

Single-channel digital LAMP multiplexing using amplification curve analysis.

Kenny Malpartida-Cardenas^1,2, Luca Miglietta^1,2, Tianyi Peng¹

¹Department of Infectious Disease, Imperial College London, London W12 0NN, UK.

Sensors & Diagnostics

|April 10, 2023

Summary

This summary is machine-generated.

Multiplexing five targets in one reaction is now possible with digital Loop-mediated isothermal amplification (LAMP) and machine learning. This advancement improves diagnostic capabilities by analyzing amplification curves for accurate target identification.

More Related Videos

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Related Experiment Videos

Last Updated: Aug 3, 2025

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Area of Science:

Molecular Biology
Biotechnology
Bioinformatics

Background:

Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique.
Current LAMP assays typically detect only one target per reaction.
Multiplexing requires additional complex methods like melting curve analysis or probes.

Purpose of the Study:

To overcome the single-target limitation of conventional LAMP assays.
To develop a method for multiplexing multiple targets in a single fluorescent channel.
To enhance the efficiency and scope of LAMP applications.

Main Methods:

Digital LAMP was employed for high-throughput amplification.
Machine learning algorithms were utilized for amplification curve analysis.
A novel approach for analyzing fluorescence data from multiplexed reactions was developed.

Main Results:

Successfully multiplexed five distinct targets within a single reaction.
Achieved a classification accuracy of 91.33% for positive amplification events.
Analyzed over 54,000 positive amplification events using the developed method.

Conclusions:

Digital LAMP combined with machine learning enables robust multiplexing.
This method significantly expands the potential of LAMP assays for complex diagnostics.
The approach offers a sensitive and accurate way to detect multiple targets simultaneously.