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Single-channel digital LAMP multiplexing using amplification curve analysis
Kenny Malpartida-Cardenas1,2, Luca Miglietta1,2, Tianyi Peng1
1Department of Infectious Disease, Imperial College London, London W12 0NN, UK.
Sensors & Diagnostics
|April 10, 2023
Abstract:
Loop-mediated isothermal amplification assays are currently limited to one target per reaction in the absence of melting curve analysis, molecular probes or restriction enzyme digestion. Here, we demonstrate multiplexing of five targets in a single fluorescent channel using digital LAMP and the machine learning-based method amplification curve analysis, resulting in a classification accuracy of 91.33% on 54 186 positive amplification events.

