Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of OsNRT2.3b transgenosis on lettuce antioxidant capacity and nitrogen metabolism under low nitrogen.

PloS one·2026
Same author

Epidemiological and Virological Characteristics of H9N2 Avian Influenza Virus in Jiangsu Province, China, 2024.

Viruses·2026
Same author

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same author

CTLA-4 and CD154 expression on peripheral T cells as diagnostic biomarkers for pulmonary tuberculosis.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Tuning the Electrode-Electrolyte Interface via Hydrogen Bond Donor Structure in Deep Eutectic Solvents for Long-Life Zinc Batteries.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Case Series of Primary Esophageal Tuberculosis.

The American journal of tropical medicine and hygiene·2026
Same journal

Reliability of A Vibration-Based Elastography Protocol For Assessing Achilles Tendon Stiffness Across Multiple Joint Angles In Elite Athletes.

Journal of visualized experiments : JoVE·2026
Same journal

Associations of Inflammatory and Coagulation Biomarkers with Kidney Injury Across Chronic and Acute Clinical Settings.

Journal of visualized experiments : JoVE·2026
Same journal

Intelligent Recommender Systems for Chinese Super League Fan Consumption Behavior Prediction.

Journal of visualized experiments : JoVE·2026
Same journal

A Battery of Quantitative Binocular Vision Tests for Adults: Testing Protocols.

Journal of visualized experiments : JoVE·2026
Same journal

Efficacy Analysis of Paiteling in Treating Persistent High-Risk Human Papillomavirus after Cervical Cancer Surgery.

Journal of visualized experiments : JoVE·2026
Same journal

Clinical Efficacy of Tissue-Bone Homeostasis Manipulation on Soft Tissue Balance and Function in Knee Osteoarthritis.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Aug 3, 2025

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

4.6K

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry.

Jing Tong1, Stefanie Rufli1, Wendy Wei-Lynn Wong2

  • 1Institute of Experimental Immunology, University of Zürich.

Journal of Visualized Experiments : Jove
|April 10, 2023
PubMed
Summary
This summary is machine-generated.

Caspase activity in cell death can be measured using fluorogenic substrates in flow cytometry assays. This method reveals the loss of apoptosis inhibitors upon tumor necrosis factor stimulation, primarily inducing apoptosis in macrophages.

More Related Videos

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

9.1K
Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
08:27

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

15.9K

Related Experiment Videos

Last Updated: Aug 3, 2025

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

4.6K
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

9.1K
Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
08:27

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

15.9K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Caspases, or cysteine proteases, are key regulators of programmed cell death, including apoptosis and pyroptosis.
  • Apoptosis is vital for development and tissue homeostasis, while pyroptosis activates the immune system via inflammasome activation and IL-1 family cytokine release.
  • Assessing caspase activity is crucial, but traditional methods using target substrates can lack sensitivity for single-cell or low-activity studies.

Purpose of the Study:

  • To develop and validate sensitive assays for measuring caspase activity in cell death.
  • To differentiate between various caspase activities using specific fluorogenic substrates.
  • To investigate the impact of tumor necrosis factor (TNF) stimulation on apoptosis inhibitors and cell death pathways.

Main Methods:

  • Utilized fluorogenic substrates for caspase activity assessment.
  • Employed both population-based and single-cell assays via flow cytometry.
  • Incorporated appropriate controls to identify specific active caspases based on amino acid sequences.

Main Results:

  • Demonstrated the utility of fluorogenic substrates for sensitive caspase activity detection.
  • Successfully identified the simultaneous loss of inhibitors of apoptosis proteins following TNF stimulation.
  • Observed that TNF primarily induces apoptosis in macrophages, distinguishing it from other cell death forms.

Conclusions:

  • Fluorogenic substrate assays provide a sensitive and versatile method for studying caspase activity in cell death.
  • These assays can distinguish specific caspases and reveal dynamic changes in apoptosis regulation.
  • TNF stimulation triggers a specific apoptotic pathway in macrophages, involving the downregulation of apoptosis inhibitors.