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Development of a Simple and Stable NanoESI Spray System Using Suction Wind from the MS Inlet

Yusuke Kawashima1, Masaki Ishikawa1, Ryo Konno1

  • 1Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.

Journal of Proteome Research
|April 10, 2023
PubMed

Keywords:
K562 cellsnanoESInanoLCspray InSpIon

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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