Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.7K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

6.7K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.8K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbial single-cell transcriptomics links gut microbiota functional states to metabolic changes in male mice.

Nature communications·2026
Same author

Hand Eczema Referred for Patch Testing: Retrospective Study in Shanghai, China From 2018 to 2023.

The Journal of dermatology·2026
Same author

Palmitoylation of PSD-95 Orchestrates Learning-Dependent Metaplasticity in the Amygdala and Fear Memory.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Risk Prediction of 2-Year Incident Cognitive Impairment in Maintenance Hemodialysis Patients: A Multicenter Prospective Cohort Study.

Kidney360·2026
Same author

Resveratrol Suppresses Lung Cancer through the NLRP3/IL-1β Signaling Axis to Improve Immune Microenvironment​.

Journal of visualized experiments : JoVE·2026
Same author

Behavioral and Electrophysiological Evidence for Repellency of <i>Phytolacca americana</i> (Pokeweed) Fruit Extract in <i>Plutella xylostella</i>.

Insects·2026

Related Experiment Video

Updated: Aug 3, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.6K

Sirtuin1-p53: A potential axis for cancer therapy.

Jia-Yi Yin1, Xin-Tong Lu1, Meng-Ling Hou1

  • 1College of Pharmaceutical Sciences, Southwest University, Chongqing, China.

Biochemical Pharmacology
|April 10, 2023
PubMed
Summary

Sirtuin1 (SIRT1) and p53 interactions are crucial in cancer. SIRT1 can act as both a tumor promoter and suppressor, depending on cellular context, influencing cancer development and treatment strategies.

Keywords:
ApoptosisCancerDeacetylatesP53SIRT1

More Related Videos

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

8.3K
Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
13:18

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

1.3K

Related Experiment Videos

Last Updated: Aug 3, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.6K
Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

8.3K
Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
13:18

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

1.3K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Oncology

Background:

  • Sirtuin1 (SIRT1), a NAD+-dependent deacetylase, regulates critical cellular processes like genome stability, proliferation, and apoptosis.
  • P53, a key tumor suppressor, is a non-histone target of SIRT1, with SIRT1 modulating its transcriptional activity.

Purpose of the Study:

  • To review the complex interactions between SIRT1 and p53 in cancer.
  • To discuss the dual role of SIRT1 (promoter vs. suppressor) in tumorigenesis.
  • To explore the therapeutic potential of targeting the SIRT1-p53 axis in cancer treatment.

Main Methods:

  • Literature review of recent evidence on SIRT1-p53 interactions.
  • Analysis of cellular events influenced by SIRT1 and p53.
  • Overview of current knowledge on SIRT1-p53 signaling in cancer.

Main Results:

  • SIRT1 deacetylates p53, inhibiting its transcriptional activity and affecting tissue homeostasis.
  • The role of SIRT1 in cancer is context-dependent, varying with cell type and localization, acting as both a tumor promoter and suppressor.
  • The SIRT1-p53 axis is central to tumorigenesis and cancer-associated cellular events.

Conclusions:

  • The contradictory roles of SIRT1 highlight the importance of cellular context in its function.
  • Understanding the SIRT1-p53 pathway is vital for cancer research.
  • Targeting the SIRT1-p53 axis offers promising therapeutic strategies for cancer treatment.