Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
DNA Bacteriophages01:26

DNA Bacteriophages

72
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
72
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

60
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
60

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-resolution genomic analysis reveals abundant mosaic outcomes of bacterial natural transformation independent of MutS-mediated mismatch repair.

mBio·2026
Same author

RNAP-seq: <i>in vitro</i> genome-scale transcription reveals preferential RNA polymerase pausing on <i>Clostridioides difficile</i> antisense DNA.

bioRxiv : the preprint server for biology·2026
Same author

Physics-constrained neural ordinary differential equation models to discover and predict microbial community dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Metabolic interplay drives population cycles in a cross-feeding microbial community.

Nature communications·2025
Same author

Physics-constrained neural ordinary differential equation models to discover and predict microbial community dynamics.

bioRxiv : the preprint server for biology·2025
Same author

Decoding the role of the arginine dihydrolase pathway in shaping human gut community assembly and health-relevant metabolites.

Cell systems·2025

Related Experiment Video

Updated: Aug 3, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.4K

Programming bacteria for multiplexed DNA detection.

Yu-Yu Cheng1, Zhengyi Chen1, Xinyun Cao1

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.

Nature Communications
|April 10, 2023
PubMed
Summary
This summary is machine-generated.

Researchers engineered Bacillus subtilis bacteria to create living DNA sensors. These sensors detect specific DNA sequences from various species, including pathogens, without needing DNA extraction, enabling broad applications.

More Related Videos

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.3K
Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

19.1K

Related Experiment Videos

Last Updated: Aug 3, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.4K
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.3K
Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

19.1K

Area of Science:

  • Synthetic Biology
  • Microbiology
  • Biosensing

Background:

  • DNA serves as a universal signal for living organisms.
  • Developing efficient and specific DNA detection methods is crucial for various applications.

Purpose of the Study:

  • To engineer Bacillus subtilis as a cell-based DNA sensor.
  • To detect specific DNA sequences in environmental samples.
  • To enable multiplexed and DNA-extraction-free species detection.

Main Methods:

  • Engineering naturally competent Bacillus subtilis for DNA sensing.
  • Utilizing orthogonal fluorescent reporters for multiplexed detection.
  • Testing sensor specificity against diverse bacterial species and complex samples.

Main Results:

  • Developed Bacillus subtilis strains capable of detecting specific DNA sequences.
  • Achieved high specificity in identifying bacterial species, including human pathogens.
  • Demonstrated multiplexed detection and detection without prior DNA extraction.
  • Showcased the modularity and programmability of the DNA-sensing system.

Conclusions:

  • Cell-based DNA sensors using Bacillus subtilis offer a novel approach for detecting specific DNA sequences.
  • The developed system allows for specific, multiplexed, and DNA-extraction-free detection of species.
  • The modularity and simplicity of the system support diverse applications in programmable DNA sensing.