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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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HDL-Chitosan Nanoparticles for siRNA Delivery as an SR-B1 Receptor Targeted System.

Rasim Masimov1, Gülay Büyükköroğlu2

  • 1College of Pharmacy and Nutrition, University of Saskatchewan, Saskatchewan, Canada.

Combinatorial Chemistry & High Throughput Screening
|April 11, 2023
PubMed
Summary

High-Density Lipoprotein (HDL)-conjugated chitosan nanoparticles effectively deliver Bcl-2 siRNA to liver cancer cells. These nanoparticles show enhanced toxicity against cancer cells overexpressing SR-B1 receptors, offering a promising targeted therapy.

Keywords:
HDLSR-B1 receptor and ApoA-1antisense technology-gene deliverychitosan nanoparticlessilencing gene-Bcl-2 siRNAtargeting therapy-liver cancer

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Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
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Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Cancer Therapy

Background:

  • High-Density Lipoprotein (HDL) facilitates cellular interactions via ApoA-1 protein and SR-B1 receptors.
  • Overexpression of SR-B1 receptors on malignant cells presents a target for cancer therapy.
  • HDL's potential for targeted delivery to cancer cells necessitates nanoparticle formulation development.

Purpose of the Study:

  • To prepare HDL-conjugated chitosan nanoparticles for targeted delivery of genetic material.
  • To develop a novel nanocarrier system for liver cancer treatment.
  • To investigate the potential of HDL-mediated targeting of cancer cells overexpressing SR-B1 receptors.

Main Methods:

  • Isolation of HDL from healthy volunteers' blood samples.
  • Preparation of chitosan nanoparticles using the ionic gelation method.
  • Conjugation of HDL to chitosan nanoparticles loaded with Bcl-2 siRNA.

Main Results:

  • Formulations exhibited particle diameters below 250 nm with positive zeta potential.
  • HDL/chitosan nanoparticles/Bcl-2 siRNA demonstrated superior toxicity against HepG2 cells compared to controls.
  • High cellular uptake ratios (76-98%) of nanoparticles by HepG2 cells were observed.

Conclusions:

  • HDL-conjugated chitosan nanoparticles offer advantages for targeted siRNA delivery to SR-B1 receptor-overexpressing malignant cells.
  • This approach shows potential for developing effective liver cancer therapeutics.
  • The developed nanocarrier system demonstrates promising characteristics for targeted cancer gene therapy.