Related Experiment Video
Updated: Aug 3, 2025

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
Published on: October 25, 2011
Variations in protein expression associated with oral cancer
Wei Jiang1,2,1, Zhiyan He1,3,1, Youmeng Zhang4,1
1Department of Endodontics and Operative Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Background:
Differential protein expression of the oral microbiome is related to human diseases, including cancer.
Objective:
In order to reveal the potential relationship between oral bacterial protein expression in oral squamous cell carcinoma (OSCC), we designed this study.
Methods:
We obtained samples of the same patient from cancer lesion and anatomically matched normal site. Then, we used the label free quantitative technique based on liquid chromatography tandem mass spectrometry (LC-MS/MS) to analyze the bacteria in the samples of oral squamous cell carcinoma at the protein level, so as to detect the functional proteins.
Results:
Protein diversity in the cancer samples was significantly greater than in the normal samples. We identified a substantially higher number of the taxa than those detected in previous studies, demonstrating the presence of a remarkable number of proteins in the groups. In particular, proteins involved in energy production and conversion, proton transport, hydrogen transport and hydrogen ion transmembrane transport, ATP-binding cassette (ABC) transporter, PTS system, and L-serine dehydratase were enriched significantly in the experimental group. Moreover, some proteins associated with Actinomyces and Fusobacterium were highly associated with OSCC and provided a good diagnostic outcome.
Conclusion:
The present study revealed considerable changes in the expression of bacterial proteins in OSCC and enrich our understanding in this point.
Related Concept Videos
RNA Splicing
What is Gene Expression?
Cell Specific Gene Expression
Tumor Progression
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...

