Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chinmo defines the region-specific oncogenic competence in the <i>Drosophila</i> central nervous system.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Tumour-driven lipid accumulation in oenocytes reflects systemic lipid alterations.

PLoS genetics·2026
Same author

The blood-brain barrier regulates brain tumor growth through the SLC36 amino acid transporter Pathetic in Drosophila.

PLoS biology·2025
Same author

Hippo signaling regulates the nuclear behavior and DNA binding times of YAP and TEAD to control transcription.

Science advances·2025
Same author

Targeting the Hippo pathway in cancer.

Nature reviews. Drug discovery·2025
Same author

Dynamic changes in neuronal and glial GAL4 driver expression during Drosophila aging.

Genetics·2025

Related Experiment Video

Updated: Aug 3, 2025

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
08:47

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

5.0K

Dedifferentiation-derived neural stem cells exhibit perturbed temporal progression.

Kellie Veen1,2,3, Phuong-Khanh Nguyen1,2, Francesca Froldi1,2

  • 1Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.

EMBO Reports
|April 11, 2023
PubMed
Summary

Mature cells can revert to a stem cell-like state, forming ectopic neural stem cells (NSCs). These ectopic NSCs exhibit impaired temporal progression, leading to an imbalance in neuronal and glial cell production.

Keywords:
Drosophiladedifferentiationneuroblasttemporal transcription factorsterminal differentiation

More Related Videos

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

10.1K
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
10:24

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

10.4K

Related Experiment Videos

Last Updated: Aug 3, 2025

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
08:47

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

5.0K
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

10.1K
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
10:24

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

10.4K

Area of Science:

  • Developmental biology
  • Neuroscience
  • Cell biology

Background:

  • Dedifferentiation reverts mature cells to a stem cell-like state, altering gene expression.
  • Misexpression of multipotency factors can induce ectopic neural stem cells (NSCs).
  • The differentiation potential and temporal control of ectopic NSCs remain largely uncharacterized.

Purpose of the Study:

  • To investigate the differentiation capacity and temporal control of ectopic neural stem cells (NSCs) induced by Deadpan (Dpn) expression.
  • To determine if these ectopic NSCs produce appropriate numbers and types of progeny.
  • To understand the molecular mechanisms underlying temporal fate decisions in ectopic NSCs.

Main Methods:

  • Induction of ectopic neural stem cells (NSCs) using the bHLH transcription factor Deadpan (Dpn).
  • Analysis of temporal transcription factor (tTF) expression, including Sloppy-paired 1/2 (Slp).
  • Assessment of neuronal (Twin of eyeless - Toy) and glial (Reversed polarity - Repo) cell production.
  • Chromatin immunoprecipitation to analyze Dpn binding at temporal transcription factor loci.
  • Manipulation of the temporal series and cell cycle to restore differentiation.

Main Results:

  • Ectopic NSCs induced by Dpn fail to progress through temporal stages, indicated by sustained Sloppy-paired 1/2 (Slp) expression.
  • This temporal defect results in an overproduction of Twin of eyeless (Toy)-positive neurons and a deficit of Reversed polarity (Repo)-positive glial cells.
  • Dpn binding is enriched at mid-temporal transcription factor loci and depleted at early- and late-temporal loci, correlating with the observed fate preference.
  • Restoring the temporal progression or manipulating the cell cycle re-establishes neuronal diversity and timely differentiation.

Conclusions:

  • Dpn-induced ectopic neural stem cells (NSCs) exhibit a blocked temporal progression, favoring a mid-temporal neuronal fate.
  • The binding preference of Dpn to specific temporal transcription factor loci underlies this fate bias.
  • Re-establishing temporal control mechanisms can rescue the differentiation potential of these ectopic NSCs, highlighting the importance of temporal regulation in neurogenesis.