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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

7.1K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Related Experiment Video

Updated: Aug 3, 2025

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

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Recent advances in single-cell subcellular sampling.

Annie Sahota1, Anthony Monteza Cabrejos1, Zoe Kwan1

  • 1Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. alex.ivanov@imperial.ac.uk.

Chemical Communications (Cambridge, England)
|April 11, 2023
PubMed
Summary
This summary is machine-generated.

New minimally invasive tools extract contents from living cells for detailed analysis without lysis. These advancements enable dynamic single-cell studies, advancing spatial transcriptomics and personalized medicine.

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Related Experiment Videos

Last Updated: Aug 3, 2025

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Single-cell analysis traditionally requires cell lysis, limiting dynamic studies and preserving cellular microenvironments.
  • Previous methods for single-cell manipulation often impacted cell viability and natural conditions.

Purpose of the Study:

  • To review key technological advances in minimally invasive single-cell content extraction.
  • To highlight applications, limitations, and future directions for these novel techniques.
  • To explore the potential impact on spatial transcriptomics and personalized medicine.

Main Methods:

  • Profiling of individual cell omics using advanced single-cell technologies.
  • Minimally invasive tools that probe and remove cellular contents from living cells.
  • Analysis of cellular contents while maintaining cell viability and native microenvironment.

Main Results:

  • Novel methods enable content extraction from living cells, preserving viability and microenvironment.
  • These techniques offer high intracellular spatial resolution for dynamic single-cell studies.
  • Current limitations and recent applications of these emerging technologies are discussed.

Conclusions:

  • Minimally invasive single-cell content extraction represents a significant advancement over traditional lysis methods.
  • These technologies hold promise for comprehensive single-cell tracking, revealing subcellular mechanisms.
  • Future expansion could accelerate the discovery of therapeutic targets and treatments, transforming personalized medicine.