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Related Concept Videos

Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Methods of Nuclear Reprogramming01:24

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Reproductive Cloning01:27

Reproductive Cloning

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Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
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Cloning of Dolly the Sheep01:08

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The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
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Related Experiment Video

Updated: Aug 3, 2025

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
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Somatic Cell Nuclear Transfer in Rabbits.

Pengxiang Qu1,2, Wenbin Cao1,2, Enqi Liu3,4

  • 1Laboratory Animal Center, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.

Methods in Molecular Biology (Clifton, N.J.)
|April 11, 2023
PubMed
Summary
This summary is machine-generated.

Somatic cell nuclear transfer (SCNT) technology can reprogram cells, making it valuable for research and agriculture. This chapter details a specific SCNT protocol for producing live cloned rabbits.

Keywords:
CloningEmbryoNuclear transplantationOryctolagus cuniculusRabbitSomatic cell nuclear transfer

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Area of Science:

  • Developmental Biology
  • Reproductive Technologies
  • Genetics

Background:

  • Somatic cell nuclear transfer (SCNT) is a key technology for cell reprogramming.
  • SCNT holds significant potential in developmental biology, biomedical research, and agriculture.
  • Rabbit cloning via SCNT, especially with transgenesis, can advance disease modeling and biopharmaceutical production.

Purpose of the Study:

  • To present a detailed protocol for somatic cell nuclear transfer (SCNT) in rabbits.
  • To facilitate the production of live cloned rabbits using SCNT.

Main Methods:

  • Detailed description of the established SCNT protocol for rabbit cloning.
  • Focus on the technical procedures and critical steps for successful SCNT in rabbits.

Main Results:

  • Successful production of live cloned rabbits utilizing the described SCNT protocol.
  • Demonstration of the efficacy of the SCNT method for rabbit cloning.

Conclusions:

  • The presented SCNT protocol is effective for producing live cloned rabbits.
  • This methodology supports the application of rabbit cloning in various research and agricultural fields.