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Updated: Aug 3, 2025

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Published on: July 6, 2022
Production of Water Buffalo SCNT Embryos by Handmade Cloning
Prabhat Palta1, Naresh L Selokar1, Manmohan S Chauhan1
1Animal Biotechnology Centre, National Dairy Research Institute, Karnal, India.
Abstract:
Cloning by somatic cell nuclear transfer (SCNT) involves the transfer of a somatic nucleus into an enucleated oocyte followed by chemical activation and embryo culture. Further, handmade cloning (HMC) is a simple and efficient SCNT method for large-scale embryo production. HMC does not require micromanipulators for oocyte enucleation and reconstruction since these steps are carried out using a sharp blade controlled by hand under a stereomicroscope. In this chapter, we review the status of HMC in the water buffalo (Bubalus bubalis) and further describe a protocol for the production of buffalo-cloned embryos by HMC and assays to estimate their quality.
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