Understanding the driving force for cell migration plasticity

Cell migration is a complex phenomenon. Not only do different cells migrate in different default modes, but the same cell can also change its migration mode to adapt to different terrains. This complexity has riddled cell biologists and biophysicists for decades in that, despite the development of many powerful tools over the past 30 years, how cells move is still being actively investigated. This is because we have yet to fully understand the mystery of cell migration plasticity, particularly the reciprocal relation between force generation and migration mode transition. Herein we explore the future directions, in terms of measurement platforms and imaging-based techniques, to facilitate the undertaking of elucidating the relation between force generation machinery and migration mode transition. By briefly reviewing the evolution of the platforms and techniques developed in the past, we propose the desirable features to be added to achieve high measurement accuracy and improved temporal and spatial resolution, permitting us to unveil the mystery of cell migration plasticity.