A new butylphthalide from Ligusticum striatum DC

One new butylphthalide ligusticumolide H (1), together with the known butylphthalides senkyunolide I (2), senkyunolide H (3), (3 R,3'S)-3'-hydroxy-3-butylphthalide (4), (3 R)-4-hydroxy-3-butylphthalide (5) and senkyunolide C (6) was isolated from the rhizome of Ligusticum striatum DC. Their structures were determined by extensive spectroscopic analyses including HR-ESI-MS, 1D and 2D NMR, and the absolute configuration of 1 was established by NMR and ECD calculations. Their effect on NO production in IL-1β-stimulated chondrocytes was also evaluated.