Mumia quercus sp. nov., isolated from the root of Quercus variabilis

A novel endophytic actinomycete, designated strain NEAU-365^T, was isolated from the root of Quercus variabilis collected from Nanjing, Jiangsu, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-365^T belonged to the genus Mumia but clearly differs from the currently recognized species Mumia zhuanghuii Z350^T (99.31 %), Mumia xiangluensis NEAU-KD1^T (98.82 %) and Mumia flava MUSC 201^T (97.78 %). Phylogenetic tree analysis revealed that strain NEAU-365^T clustered with the type strains of the genus Mumia. The genome size was 4.1 Mbp with a DNA G+C content of 71.2 mol%. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain NEAU-365^T and those of M. zhuanghuii Z350^T(27.5 and 84.0 %), M. xiangluensis NEAU-KD1^T(23.4 and 80.9 %) and M. flava MUSC 201^T(20.9 and 77.7 %) were below the recommended thresholds for species delineation. Cells were observed to be irregular cocci shaped. The cell wall contained ll-diaminopimelic acid and the whole-cell sugars were galactose and rhamnose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and three unidentified phospholipids. The predominant menaquinone was detected as MK-9(H₄). The major fatty acids were C_16 : 0, C_18 : 0, C_18 : 1 ω9c and 10-methyl C_18 : 0. On the basis of genotypic and phenotypic differences from members of the genus Mumia, a novel species, Mumia quercus sp. nov. is proposed. The type strain is NEAU-365^T (=CCTCC AA 2021033^T=JCM 35005^T).