Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

PDE5a Inhibition Restricts Cancer Metastasis by Disrupting NPC1-Mediated Cholesterol Trafficking Through a Non-canonical cGMP-Dependent Pathway.

Cancer research·2026

Same author

When Biology Pushes Back: Lessons From HBsAg-Targeting Therapies in Hepatitis Delta.

Journal of hepatology·2026

Same author

Structural and Mechanistic Basis of F227C-Mediated Hypersusceptibility to Islatravir in HIV-1 Reverse Transcriptase.

bioRxiv : the preprint server for biology·2026

Same author

Unraveling the Mechanism of HIV-1 Hypersusceptibility to Tenofovir Imparted by Islatravir Resistance Mutations.

bioRxiv : the preprint server for biology·2026

Same author

Cell-based platforms for antiviral screening against hepatitis B virus: advantages, limitations, and future perspectives.

Antiviral research·2026

Same author

Hepatitis C virus infection dynamics, treatment, and lipid nanoparticle-mediated infection in humanized liver chimeric mouse models.

Science advances·2026

Search research articles

Related Experiment Video

Updated: Aug 3, 2025

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

An RNA-based system to study hepatitis B virus replication and evaluate antivirals.

Yingpu Yu¹, William M Schneider¹, Maximilian A Kass^1,2

¹Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.

Science Advances

|April 12, 2023

Summary

Researchers developed a novel method to initiate Hepatitis B virus (HBV) replication using synthetic RNA. This breakthrough aids in studying HBV replication and discovering new antiviral drugs by reducing background noise.

More Related Videos

A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

Related Experiment Videos

Last Updated: Aug 3, 2025

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

Area of Science:

Hepatology
Virology
Molecular Biology

Background:

Chronic Hepatitis B virus (HBV) infection affects 300 million globally, leading to liver cirrhosis and cancer.
Current treatments for chronic HBV are often ineffective, highlighting the need for new antiviral strategies.
Existing research systems for HBV replication are hampered by background signals from virus or plasmid DNA.

Purpose of the Study:

To develop an innovative method for initiating HBV replication.
To provide a cleaner system for studying multiple stages of HBV replication.
To establish a platform for identifying antiviral drug resistance mutations.

Main Methods:

Initiation of HBV replication using synthetic RNA.
Elimination of contaminating background signals from virus or plasmid DNA.
Application of the method to study HBV replication and identify drug-resistant variants.

Main Results:

Successfully initiated HBV replication using synthetic RNA, offering a novel research tool.
The method effectively reduced background noise, enabling clearer observation of HBV replication.
Demonstrated the utility of the system in identifying sequence variants conferring antiviral resistance.

Conclusions:

The synthetic RNA-initiated HBV replication system offers a significant advancement in HBV research.
This method provides a valuable tool for understanding HBV biology and developing effective antiviral therapies.
The system facilitates the discovery of drug resistance mechanisms, crucial for optimizing treatment strategies.