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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
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Related Experiment Video

Updated: Aug 3, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Lateral flow immunoassay for proteins.

Xuanxu Nan1, Li Yang2, Yue Cui1

  • 1School of Materials Science and Engineering, Peking University, Beijing 100871, PR China; First Hospital Interdisciplinary Research Center, Peking University, Beijing 100871, PR China.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|April 12, 2023
PubMed
Summary
This summary is machine-generated.

Lateral flow immunoassays (LFIA) offer a low-cost, easy-to-use method for detecting protein biomarkers in point-of-care settings. This review examines LFIA principles, components, and applications, highlighting advancements for global healthcare.

Keywords:
Device configurationLateral flow immunoassayMaterialMedical applicationProtein

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Protein biomarkers are crucial for disease diagnosis.
  • Lateral flow immunoassays (LFIA) are increasingly important for in vitro diagnostics due to their cost-effectiveness and ease of use, particularly in point-of-care applications.
  • Challenges persist in material selection and component integration for optimal signal detection in LFIAs.

Purpose of the Study:

  • To review the principles and components of lateral flow immunoassays for protein biomarker detection.
  • To examine the medical applications and commercial status of LFIAs.
  • To highlight methodologies for developing advanced LFIAs to improve global healthcare.

Main Methods:

  • Literature review of existing research on lateral flow immunoassay technology.
  • Analysis of biomaterials, labeling methods, and device integration strategies.
  • Examination of current medical applications and commercial landscape of LFIAs.

Main Results:

  • LFIAs utilize specific binding interactions for protein biomarker detection.
  • Key components include nitrocellulose membranes, sample pads, conjugate pads, and absorbent pads.
  • Various labeling methods (e.g., gold nanoparticles, latex beads) enhance signal visibility.
  • Significant progress has been made in materials science and assay development.

Conclusions:

  • Lateral flow immunoassays are a vital tool for accessible disease diagnosis.
  • Overcoming current challenges in material selection and integration will further enhance LFIA performance.
  • Continued development of LFIAs holds significant promise for advancing global healthcare and diagnostics.