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Related Experiment Video

Updated: Aug 3, 2025

Laser Micro-Irradiation to Study DNA Recruitment During S Phase
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NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA Damage in Prostate Cancer.

Chun-Lung Chiu1, Caiyun G Li2, Erik Verschueren3

  • 1Department of Urology, Stanford University School of Medicine, Stanford, CA 94305, USA.

International Journal of Molecular Sciences
|April 13, 2023
PubMed
Summary
This summary is machine-generated.

Nuclear matrix protein NUSAP1 interacts with ILF2 to maintain RNA-DNA hybrids (R-loops) and DNA damage response in prostate cancer, offering a potential therapeutic target.

Keywords:
DHX9DNA damageILF2NUSAP1R-loopprostate cancer

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Area of Science:

  • Molecular Biology
  • Cancer Research
  • Proteomics

Background:

  • Nuclear matrix protein NUSAP1 is a prognostic biomarker in prostate cancer, linked to invasion and metastasis.
  • NUSAP1 expression is regulated by E2F1 and influences cancer progression.

Purpose of the Study:

  • To identify proteins interacting with NUSAP1 and elucidate its biological functions.
  • To investigate NUSAP1's role in R-loop maintenance and DNA damage response.

Main Methods:

  • Affinity purification coupled with mass spectrometry to identify NUSAP1 interactors.
  • Co-immunoprecipitation and confocal microscopy to verify protein and RNA/DNA hybrid interactions.
  • Depletion studies to assess the functional impact of NUSAP1 and ILF2.

Main Results:

  • Identified 85 unique proteins interacting with NUSAP1, including ILF2 and DHX9.
  • Uncovered a role for NUSAP1 in maintaining R-loops and DNA damage response via ILF2 interaction.
  • Demonstrated that NUSAP1 and ILF2 mRNA levels correlate with poor outcomes in prostate adenocarcinoma.

Conclusions:

  • NUSAP1 interacts with ILF2 to regulate R-loop formation and DNA damage response.
  • The microtubule and charged helical domains of NUSAP1 are crucial for these interactions.
  • NUSAP1 and ILF2 represent potential therapeutic targets for prostate cancer.