Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Immunology

Background:

• Graphene oxide (GO) possesses unique chemical-physical and biological properties, including anti-inflammatory effects.
• Inflammation plays a crucial role in various biological processes and diseases.

Purpose of the Study:

• To evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs) enriched with graphene oxide (GO).
• To investigate the modulation of the Toll-like receptor 4 (TLR4)/MyD88/NFκB p65/NLRP3 inflammatory pathway by GO-enriched PPSTBs.

Main Methods:

• In vitro study using primary human gingival fibroblasts (hGFs) stimulated with lipopolysaccharide from Escherichia coli (LPS-E).
• Assessment of inflammatory marker gene and protein expression using real-time PCR, western blotting, and immunofluorescence.
• Evaluation of hGFs morphology and adhesion on PPSTBs-GO constructs via microscopy and real-time PCR.

Main Results:

• Both concentrations of GO in PPSTBs-GO constructs significantly decreased inflammatory marker expression in LPS-E treated hGFs.
• GO enrichment did not adversely affect hGFs morphology or adhesion on the suture materials.
• The study demonstrated modulation of the TLR4/MyD88/NFκB p65/NLRP3 pathway by PPSTBs-GO.

Conclusions:

• Polypropylene suture threads buttons enriched with graphene oxide exhibit significant anti-inflammatory properties.
• GO-enhanced PPSTBs modulate the TLR4/MyD88/NFκB p65/NLRP3 inflammatory pathway.
• These findings suggest potential applications for GO-modified biomaterials in managing inflammatory conditions.